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Sunday, 25 June
23:59
Why a Germ Expert Is Begging You To Remove the Foil or Plastic Seal Under the Lid of Your Containers After Opening Healthy Holistic Living
If you frequent Reddit or engage in household debates on food storage, youve likely encountered the controversy surrounding foil or plastic seals on food products like yogurt and sour cream. Should these seals be removed or retained for additional coverage after opening? One couple debated this question for an impressive ten years before a directive from the very product in question solved their quandary. The husband found the unexpected resolution under the contentious disposable seal, which was imprinted with a manufacturers instruction: To prolong freshness, completely remove and discard this foil seal.
This straightforward message from the product manufacturer may solve the debate, but it still leaves us wondering about the original purpose of the seals. Moreover, how can leaving these seals on an opened container compromise the freshness of its contents? In pursuit of these answers, we consulted two renowned germ experts.
Understanding the Purpose of Foil or Plastic Seals on Food Containers
According to Jason Tetro, a respected microbiologist and author of the book The Germ Files, there are two primary reasons for the existence of these seals. Firstly, it helps to increase shelf lifethe container is usually flushed with nitrogen before sealing, which reduces the amount of oxygen inside, he explains. Nitrogen flushing is a common technique used by food manufacturers to prolong the shelf-life of their products. The reduction of oxygen inside the container is intended to suppress the growth of aerobic bacteria and fungi, which can cause food to spoil.
Secondly, these seals serve as a protective barrier for the containers contents. It helps to prevent the introduction of chemicals and microbes that could spoil the products and/or cause illnesses, states Tetro.
Adding further clarity to this matter, microbial ecologist Jack A. Gilbert, Ph.D., a professor at the University of California San Diego, elaborates on the importance of the seal. He describes it as a safety and quality mechanism. It works by creating a barrier between the product and the external environment while also providing assurance to consumers that the product has not been tampered with prior to purchase.
Removing the Seal Post-Opening: A Crucial Practice for Food Safety
In casual parlance, breaking the seal refers to the first bathroom visit while c...
23:02
McDonalds Fry Scandal Unveiled: The Not-So-Vegetarian Secret Behind the Worlds Most Iconic Fries Healthy Holistic Living
This might leave you in a bit of a conundrum.
Popular TikToker, Jordan Howlett, famous for his intriguing fast food secrets, has dropped a bombshell revelation on his 9 million followers: McDonalds world-renowned fries might not be as vegetarian-friendly as we thought. This potentially game-changing information, targeting one of the key aspects of McDonalds global allure, has shaken the fast-food world to its core.
The Controversial Ingredient: Natural Beef Flavor
Howlett, 26, points out an ingredient named natural beef flavor thats found on the companys US online menu. He asserts that this is the reason why McDonalds fries taste different from everybody elses fries.
McDonalds cooks their fries with beef flavoring mixed within their vegetable oil, the San Diego-based TikToker explained in a recent TikTok video that has amassed over 9.6 million views. He suggests that this ingredient is what makes McDonalds fries irresistibly tasty but may pose a significant problem for vegetarians and those with specific dietary restrictions.
@jordan_the_stallion8 #stitch with @youraveragetechbro #fyp original sound Jordan_The_Stallion8
Howlett, upon discovering the use of beef flavoring in the fries,
was rather intrigued. I thought it was incredibly interesting, he
shared. He thought that the public, particularly those with dietary
restrictions, should be aware of this information. Whether it
contains meat or not doesnt really change my view on the McDonalds
fries. I still enjoy them, he added.
McDonalds Response: Beef Flavorings Recipe
McDonalds, on its website, confirms the use of beef flavoring in their fry prep...
22:17
This Widely Used Herb in Ayurveda Has Multiple Health Benefits Including Cancer Prevention, Inflammation Reduction, and Blood Purification Healthy Holistic Living
Rooted deep within the traditional medicinal practices of India lies an extraordinary herb, extensively used and profoundly respected for millennia: the Neem, scientifically known as Azadirachta indica. From disease prevention and longevity to overall bodily wellness, neem has repeatedly proven its extraordinary efficacy. Its numerous, scientifically backed health benefits span from potent anti-cancer and anti-inflammatory effects to blood purification, liver health, and beyond. This comprehensive guide will delve into the fascinating world of neem, offering an enriching insight into its myriad health benefits, as well as sharing useful tips on its usage.
The Neem Herb: A Potent Life Enhancer
Boasting a long history of therapeutic use, Neem has continued to pique the interest of both traditional herbal enthusiasts and modern scientists alike. The ancient Ayurvedic medicinal system recognized its versatile healing capabilities, which modern research has continued to corroborate and elaborate upon. Below, we will embark on an exploration of the numerous health benefits of neem.
1. Anti-Cancer Properties
Neems prowess as a potential natural anti-cancer agent has received significant attention within the scientific community. Various studies, such as this one, have underscored its potent anticancer properties. Neem extracts have demonstrated the capacity to inhibit the growth and spread of cancer cells, notably by inducing apoptosis the process of programmed cell death that prevents their uncontrolled proliferation. Beyond this, neem also enhances the immune systems ability to target and destroy cancer cells, making it an invaluable adjunctive therapy alongside conventional cancer treatments.
2. Anti-Inflammatory Properties
Chronic inflammation is a common denominator underlying numerous
health conditions, from cardiovascular diseases and arthritis to
diabetes. Neem exhibits remarkable
anti-inflammatory properties that can alleviate symptoms and
promote overall wellness. Active compounds in neem, like nimbin and
nimbidin, inhibit pro-inflammatory enzymes and cytokines, thereby
reducing inflammation and relieving discomfort. Integrating neem
into your daily routine can offer a natural, effective way to
manage inflammatory conditions.
...
21:37
N-Acetyl Cysteine: NAC Supplement Powerful Antioxidant Healthy Holistic Living
Research suggests that N-acetyl cysteine (NAC) the supplement form of the semi-essential amino acid L-cysteine is an effective, safe and low-cost treatment option for a range of conditions, including those that are both acute and chronic.
What are the benefits of taking NAC? Not only has been it been used for three decades as an effective mucolytic agent (mucous-thinner), but it also has uses including reducing inflammation, protecting the liver, and preventing/treating drug toxicity, insulin resistance, infertility due to PCOS and more.
What Is NAC?
N-acetyl cysteine, the supplement form of cysteine, is an amino acid that helps the body create and use protective antioxidants.
While cysteine is found in many high-protein foods, NAC is only obtained from taking supplements or prescription drugs.
Because the human body can create some NAC from other amino acids, particularly l-methionine and l-serine, it is considered to be a semi-essential amino acid. That means there isnt a daily requirement of NAC that you must get from your diet, but getting higher amounts can benefit some people.
Benefits
1. Has Antioxidant Effects and Helps Form Glutathione
NAC is involved in the regulation of the glutamatergic system. In other words,...
10:00
The Importance of Omega-3 for Cell Membrane Functionality Articles
In this interview, Nils Hoem, Ph.D., a research scientist with Aker Biomarine, the largest krill oil company in the world takes a deep dive into omega-3s and the crucial role they play in the health and functionality of your cell membranes.
In my academic life, I spent the first 20 years as a researcher at the University of Oslo. I got my master's and doctorate from the University of Oslo in pharmacology, and was an associate professor there ... Fifteen years ago, I came ... to work for Aker Biomarine ... as the chief scientist, but I'm, by heart and mind, really, a pharmacologist, Hoem says.
The Importance of Membranes
As explained by Hoem, the cellular membrane is the universal surface onto which, and into which, the cellular machinery is assembled. Very little in the cell just floats around. It's a very complex structure and the integrity of membranes is absolutely vital for the function of the cell, he says.
The cell membrane also hosts response elements and almost all signaling, except for hormones. Almost all short path signaling begins in the membrane. Your mitochondria also have an inner and outer membrane, and the function of these are also crucial for health.
Membranes are lipid structures made of phospholipids and other constituents. Inside we find phosphatidylcholine and phosphatidylethanolamine, two ampholytic phospholipids, meaning they have a polar end and nonpolar end.
The food you eat provides the raw material substrate that is then assembled into the mitochondrial and cellular membranes, which is why the type of fats you consume is so important.
Omega-6 Competes Against Omega-3
As explained by Hoem, there are two polyunsaturated fats (PUFAs) that are considered to be essential in conventional medicine. One of them is the omega-6 linoleic acid (LA), which is an 18-carbon molecule. Although the level of LA needed is likely significantly lower than suggested, it is a moot point as virtually consuming 10 times the suggestion. The other is omega-3 alpha-linolenic acid (ALA), which also has 18 carbons.
Your body cannot make these fats, so you must get them from your diet. That said, since LA is found in nearly every food, and you need very small amounts, its virtually impossible to become deficient in LA.
Others, such as the omega-3 EPA and DHA, which have 20 and 22 carbons respectively, can be synthesized in your body, provided you have enough available delta-6-desaturase, an enzyme responsible for their conversion.
The problem is that there's competitive inhibition for that enzyme, so when you have 10-fold (1,000%) more omega-6 in your system, then th...
How the Censorship Industry Works, and How We Can Stop It Articles
In this video, I interview Mike Benz, executive director for the Foundation for Freedom Online. Benz started off as a corporate lawyer representing tech and media companies before joining the Trump administration, where he worked as a speech writer for Dr. Ben Carson, the former U.S. Secretary of Housing and Urban Development (HUD) and President Trump.
He also advised on economic development policy. He then joined the State Department as Deputy Assistant Secretary for International Communications and Information Technology. There, he ran the cyber desks at state, meaning all things having to do with the internet and foreign policy.
This is toward the end of 2020, which was a really fascinating time to witness the merger, in many respects, of big government and big tech companies themselves, he says. I had grown up, I think, like many Americans, with a belief that the First Amendment protected you against government censorship.
The terms of engagement that we had enjoyed from 1991, when the worldwide web rolled out, until 2016, the election in the U.S. and Brexit in the U.K., which is, really, the first political event where the election was determined, in many respects, by momentum on the internet.
There was that 25-year golden period where the idea of being censored by a private sector company, let alone the government, was considered something, to me, very deeply anathema to the American experience.
What I witnessed at the State Department because I was at the desk, basically, that Google and Facebook would call when they wanted favors abroad, when they wanted American protection or American policies to preserve their dominance in Europe, or in Asia or in Latin America.
And the U.S. government was doing favors for these tech companies while the tech companies were censoring the people who voted for the government. It was a complete betrayal of whatever social contract typically underlies the public-private partnership.
The Internet Was Founded by the National Security State
Ostensibly, the rapid expansion of censorship started post-2016, but you can make a strong argument that the internet was never intended to remain free forever. Rather, the intention for it to be used as a totalitarian tool was likely baked in from the start when the national security state founded it in 1968.
The worldwide web, which is the user interface, was launched in 1991, and my suspicion is that the public internet was seeded and allowed to grow in order to capture and make the most of the population dependent upon it, knowing that it would be...
Hallmarks of Alzheimer's Are Stimulated by This Substance Articles
Editor's Note: This article is a reprint. It was originally published March 10, 2019.
Boyd Haley, Ph.D., is a chemist specializing in the development of chemicals to chelate toxic metals, both from the environment and the human body. I had the opportunity to interview Haley (above) at the 2018 Academy of Comprehensive Integrative Medicine (ACIM) conference in Orlando.
Haleys Ph.D. is in chemistry and biochemistry. He conducted research funded by the National Institutes of Health (NIH) for 25 years at the University of Wyoming and at the University of Kentucky. Early in his career, he developed a biochemical detection system called nucleotide photoaffinity labeling and has published studies on its usage.1 Haley explains:
"I took ATP and made it radioactive, which isn't a big feat. But then I attached to that a molecule that would explode when it hit a photon of light. When it exploded, it made a very reactive intermediate that had a half-life of something like 10-12 or 10-13 seconds.
If ATP was bound to a protein, such as sodium potassium ATP [and] you hit it with light, it would form a covalent bond at the binding site of ATP on the enzyme it was interacting with
You could use these kinds of probes to see the difference between the ATP, guanosine diphosphate (GDP), cyclic adenosine monophosphate (AMP) and nicotinamide adenine dinucleotide (NADH) all these binding proteins, to see how the energetics of the cell was changing."
Haley's Alzheimer's Research
He later took a position with the Alzheimer's Center, a research center for Alzheimer's disease, where he collaborated with a former graduate student of his. The NIH funded their research for five years, which used Haley's technology to assess the differences of ATP, GDP and cyclic AMP binding proteins in normal brains versus those with Alzheimer's disease.
"There were dramatic differences," he says. For example, the enzyme creatine kinase, which is a fundamental enzyme, is 98% inhibited in Alzheimer's patients. They also discovered that tubulin a major brain protein that holds an axon in its extended form and controls the growth direction of axons and dendrites is inhibited by more than 80%.
In 1989, he published the paper2 "Aberrant Guanosine Triphosphate-Beta-Tubulin interaction in Alzheimer's disease" in the Annals of Neurology, stating that "These results support the hypothesis that microtubule formation is abnormal in brains affected by Alzheimer's disease."
...08:45
Geoengineering Watch Global Alert News, June 24, 2023, #411 Geoengineering Watch
Dane Wigington GeoengineeringWatch.org Extreme
hail events have just hammered Colorado, Texas, Kansas and parts of
the Northeastern US, climate engineering and chemical ice
nucleation cloud seeding operations are core to the equation.
Temporary toxic surface cooldowns are a constant objective of the
geoengineers, the resulting consequences aren't a consideration for
them. Snow fell this week in
08:01
Survey Says Age of Autism The Rebel Alliance!
By Cathy Jameson
I love our quieter summer schedule. The alarm clock isnt screaming at us to wake up early. The kids arent rushing here and there. And Im able to sit still a bit longer to catch up on some things Ive had to put on hold. With the extra quiet time, Ive started to sift through my email to get rid of old messages. Every few days, I get rid of promotions that have expired and also delete school notices that are now irrelevant. In sitting down late last week to get sort through a handful of older messages, I saw that I had a new one. I made sure not to delete it:
Dear Cathy,
Over the course of the COVID-19 pandemic, Childrens Health Defense (CHD) experienced considerable growth in its membership, as people sought information about vaccine efficacy and safety, and community support in the face of restrictive government and workplace policies such as vaccine mandates. To our knowledge, little research has been done on the experiences and consequences of these policies for overall wellbeing, especially for people who declined vaccination.
If you subscribe to CHD, you probably saw that announcement, too. Coupled with it is the chance to take a survey to better understand our subscribers experiences with vaccine mandates since the beginning of the pandemic. The survey will take about 510 minutes to complete.
I have friends who knew to be skeptical of what our government was saying and doing early on during the pandemic. Some had never been vocal about vaccines. Now, they are being more than vocal. Theyre saying never again.
Never again will they be duped or talked into taking something so risky.
Never will they risk their job, their health, or their freedom.
Never.
Early on, when people would ask me where they could look for information about what was going on, I made sure to share the CHD webpage. Look here for some helpful resources. Check out what this group is working on. Read up on who the talking...
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Saturday, 24 June
21:00
Weekend reads: A fraudster is sentenced to prison; how another reinvented himself; RFK Jr.s retraction, redux Retraction Watch
Would you consider a donation to support Weekend Reads, and our daily work?
The week at Retraction Watch featured:
- Plagiarism scandal engulfs high-profile academic in Latvia
- Journalism with impact: The work of Retraction Watch
- Financial advisor failed to disclose he had sued the organization his paper criticized
- In a Tipsters Note, a View of Science Publishings Achilles Heel
- Deplorable: Imaging journal to retract nearly 80 papers for compromised peer review
- Paper retracted more than eight months after author admitted to plagiarism
Our list of retracted or withdrawn COVID-19 papers is up to more than 300. There are now 41,000 retractions in our database which powers retraction al...
20:00
Psychiatrys Denial of the Horrors of Tardive Dyskinesia Mad In America
Even though tardive dyskinesiaand also akathisia, which it resemblesare serious and horrible harms of psychiatric drugs, they are often overlooked or deliberately ignored by psychiatrists.
It took psychiatry 20 years to recognise tardive dyskinesia as an iatrogenic illness, even though it affects about 4-5% of the patients receiving neuroleptics per year. Poul Leber from the FDA concluded in 1984 that, over a lifetime, all patients might develop tardive dyskinesia. Three years later, the president of the American Psychiatric Association said on an Oprah Winfrey show that tardive dyskinesia was not a serious or frequent problem.
Neurologists are much better at spotting tardive
dyskinesia than psychiatrists and the same applies to researchers.
Researchers found tardive dyskinesia in 10 of 48 patients
consecutively admitted with acute psychosis and treated for at
least a week with neuroleptics, while the psychiatrists only made
this diagnosis in one of them.
Akathisia is also common but is also often missed, ignored or misinterpreted. In the same study, the researchers diagnosed akathisia in 27 patients, the clinicians only in 7. In a community sample of patients with schizophrenia, the prevalence was 19%.
Tardive dyskinesia is an involuntary drug-induced movement disorder characterised by repetitive purposeless movements. It can have many manifestations, including fa...
17:37
Steve Kirsch and Brandolinis law Science-Based Medicine
The amount of energy needed to refute bullshit is an order of magnitude bigger than that needed to produce it.
The post Steve Kirsch and Brandolinis law first appeared on Science-Based Medicine.11:19
13 Chemical Threats to Remove from Your Home GreenMedInfo
Visit our Re-post guidelines
While people are aware of the health risk caused by outdoor air pollution, few may consider that indoor air quality can also have a negative impact on their health. While many of these products are commonly used in the home there are healthier options available.
10:00
Oral Myofacial Therapy Articles
Editor's Note: This article is a reprint. It was originally published April 7, 2013.
Orofacial myofunctional therapy is a profoundly useful treatment that may help treat the symptoms of a wide variety of health issues, from opening airways to headaches, temporomandibular joint disorders (TMJ), to poor digestion, just to name a few. It may be the most profound therapy available for the treatment of mild to moderate sleep apnea, which is a pervasive problem that affects many.
Myofunctional therapy is the "neuromuscular re-education or repatterning of the oral and facial muscles."1
The therapy includes facial and tongue exercises and behavior modification techniques to promote proper tongue position, improved breathing, chewing, and swallowing. Proper head and neck postures are also addressed. There are good studies to substantiate that it may resolve jaw problems and orthodontic relapse working in a multidisciplinary team.
It may also be an alternative or adjunctive treatment to facial plastic surgery, to help get rid of lines and saggy facial muscles, which is exciting. In fact, in Brazil, some myofunctional therapists work with plastic surgeons.
Joy Moeller, whom I had the good fortune of meeting as a result of an earlier interview with Carol Vander-Stoep (author of the book "Mouth Matters; How Your Mouth Ages Your Body and What YOU Can Do About It"), is a leading expert in this form of therapy in the U.S.
At the end of that interview with Vander-Stoep, we discussed the issue of being "tongue tied," and she happened to look into my mouth. A large population of Americans are estimated to have health problems related to poor orofacial function, and the vast majority have no idea their problems stem from the dysfunction of their mouth (primarily the tongue), breathing habits, and forward head and neck muscle function. It turns out I was one of them.
As a result, I got on a treatment plan with Moeller, who is a premier expert on myofunctional therapy in the U.S. We did regular Skype sessions for several months, and not only did I make great progress in resolving my tongue tie, but it had a profoundly beneficial effect on my posture and amount of deep sleep as well.
Of course, adult problems associated with tongue-tie are best prevented in infancy beginning with ensuring the normal duration of breastfeeding. However, tongue-tie often goes undetected by OBGYNs and pediatricians who, having been educated in a bottle-feeding culture, fail to connect the related breastfeeding issues.
When feeding issues are present, such as recurring maternal pain, thrush or mastitis, poor latch, inefficient emptying of the breast,...
Throughout the pandemic and beyond, youve likely heard top political figures and Big Tech leaders talking about the opportunity to reset and build back better. Founder and executive chairman of the World Economic Forum (WEF) Klaus Schwab is often said to be the mastermind behind this and is credited with circulating the idea of The Great Reset, of which build back better is an integral part.
However, there are other nefarious players who may have even more power, namely the newly crowned King Charles III.
When The Great Reset was officially launched in 2020, it was not done so by Klaus Schwab or Bill Gates, but by Charles, Prince of Wales, then heir-apparent to the British throne, according to Winter Oak, an independent not-for-profit organization development agenda and a commitment to truth, freedom and social justice.1
Theyve outlined key details on why King Charles should be on your radar when it comes to the implantation of The Great Reset and Agenda 21 (Agenda for the 21st Century) the inventory and control plan for all land, water, minerals, plants, animals, construction, means of production, food, energy, information, education and all human beings in the world.
Is King Charles The Great Resetter?
In June 2020, King Charles, then The Prince of Wales, announced he was launching a new global initiative, The Great Reset, along with WEFs and His Royal Highness Sustainable Markets Initiative.2 A tweet from Clarence House stated:3
#TheGreatReset initiative is designed to ensure businesses and communities build back better by putting sustainable business practices at the heart of their operations as they begin to recover from the coronavirus pandemic.
Winter Oak explained:4
This may come as a bit of a surprise to those who see Charles as a bumbling but affable figure, who talks to his plants, loves traditional architecture, protects nature and tries to help young people get along in life. But the reality is that he is the head (or the very willing figurehead) of a vast empire of nefarious financial interests hiding hypocritically behind a facade of charitable philanthropy.
King Charles charitable enterprise spans a ...
Why Are Planes Using Pork Byproducts for Fuel? Articles
Rendered animal fats, a byproduct of raising animals on concentrated animal feeding operations (CAFOs), are commonly added to pet food and personal care products like soap and cosmetics. However, theyre also a growing part of "sustainable aviation fuels" (SAF), to the extent that theres not enough to go around.
A study conducted by Cerulogy for Transport & Environment (T&E) revealed increasing pressure on animal fat supplies as its use in biodiesel has skyrocketed 40-fold since 2006.1
8,800 Dead Pigs to Fuel One Transatlantic Flight
Animal fats are being touted as the latest sustainable biofuel, but as usage grows its already appearing to be an unsustainable fuel source. According to the T&E report, demand for animal fats for biofuel may triple from 2021 to 2030.2 However, in Europe, 46% of animal fat feedstocks are already used as biodiesel, suggesting supply wont keep up with demand.
The study calculated that to fuel one transatlantic flight from Paris to New York, 8,800 dead pigs are needed for one way. In the European Union, animal fats are categorized into three groups depending on their risk levels for disease transmission. While categories 1 and 2 can only be used for heating, category 3 can be used in a wider range of applications, including pet food, oleochemical industries and biofuel.
With biofuel creating more demand for those category 3 fats, other industries may be pushed out of the market or find products being made with animal-fat substitutes, which are usually cheaper available oils.3 Nicole Paley, deputy chief executive of UK Pet Food, told BBC:4
"These [animal fats] are really valuable ingredients for us and they are hard to replace, and they're put to good use already in a very sustainable way So actually diverting these ingredients to biofuels is actually creating another problem. It would put us in competition with the aviation industry. And when it comes to the purse strings of the aviation sector, the pet food industry would find it really difficult to compete."
Dickon Posnett from Argent Energy, which produces biodiesel from wastes, added, "If you make a big incentive for use of these lipids, animal fats, and used cooking oils, in aviation, it will inevitably take it away from other things. So if you want to increase aviation sustainability, at the expense of truck sustainability, then crack on. But that's a decision for the government to make."5
UK Considers Ban on Animal Fats in Aviatio...
06:31
Low risk of Guillain-Barr syndrome after COVID mRNA vaccines Skeptical Raptor
The Skeptical Raptor, stalking pseudoscience in the internet jungle.
New research shows that the risk of Guillain-Barr syndrome after receiving COVID-19 mRNA vaccines is the same as unvaccinated individuals.
05:44
Protective mechanism of salvianolic acid B on blood vessels. GreenMedInfo
PMID: Zhongguo Zhong Yao Za Zhi. 2023 Mar ;48(5):1176-1185. PMID: 37005801 Abstract Title: [Protective mechanism of salvianolic acid B on blood vessels]. Abstract: Salvianolic acid B(Sal B) is the main water-soluble component of Salvia miltiorrhiza Bunge. Studies have found that Sal B has a good protective effect on blood vessels. Sal B can protect endothelial cells by anti-oxidative stress, inducing autophagy, inhibiting endoplasmic reticulum stress(ERS), inhibiting endothelial inflammation and adhesion molecule expression, inhibiting endothelial cell permeability, anti-thrombosis, and other ways. In addition, Sal B can alleviate endothelial cell damage caused by high glucose(HG). For vascular smooth muscle cell(VSMC), Sal B can reduce the synthesis and secretion of inflammatory factors by inhibiting cyclooxygenase. It can also play a vasodilatory role by inhibiting Ca~(2+) influx. In addition, Sal B can inhibit VSMC proliferation and migration, thereby alleviating vascular stenosis. Sal B also inhibits lipid deposition in the subendothelium, macrophage apoptosis, thereby reducing the volume of subendothelial lipid plaques. For some atherosclerosis(AS) complications, such as peripheral artery disease(PAD), Sal B can promote angiogenesis, thereby improving ischemia. It should be pointed out that the conclusions obtained from different experiments are not completely consistent, which needs further research. In addition, previous pharmacokinetics showed that Sal B was poorly absorbed by oral administration, and it was unstable in the stomach, with a large first-pass effect in the liver. Sal B had fast distribution and metabolism in vivo and short drug action time. These affect the bioavailability and biological effects of Sal B, and the development of clinically valuable Sal B non-injectable delivery systems remains a great challenge.
05:42
Chlorogenic acid protective effects on paraquat-induced pulmonary oxidative damage and fibrosis. GreenMedInfo
PMID: J Biochem Mol Toxicol. 2023 Apr 3:e23352. Epub 2023 Apr 3. PMID: 37010041 Abstract Title: Chlorogenic acid protective effects on paraquat-induced pulmonary oxidative damage and fibrosis in rats. Abstract: Paraquat (PQ) is a widely used herbicide that can cause severe oxidative and fibrotic injuries in lung tissue. Due to the antioxidant and anti-inflammatory properties of chlorogenic acid (CGA), the present study investigated its effects on PQ-induced pulmonary toxicity. To this end, 30 male rats were randomly categorized into five groups of six. Initially, the first and third groups were treated intraperitoneally (IP) with normal saline and CGA (80mg/kg) for 28 consecutive days, respectively. The second, fourth, and fifth groups were treated with normal saline and 20 and 80mg/kg of CGA for 28 consecutive days, respectively, and received a single dose of PQ (IP, 20mg/kg) on Day 7. Then, the animals were anesthetized with ketamine and xylazine, and lung tissue samples were collected for biochemical and histological examinations. The results showed that PQ significantly increased hydroxyproline (HP) and lipid peroxidation (LPO) and decreased the lung tissue antioxidant capacity. In addition, myeloperoxidase (MPO) activity increased significantly, while glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) activity declined substantially. The administration of therapeutic doses of CGA could prevent the oxidative, fibrotic, and inflammatory effects of PQ-induced lung toxicity, and these changes were consistent with histological observations. In conclusion, CGA may improve the antioxidant defense of lung tissue and prevent the spread of inflammation and the development of PQ-induced fibrotic injuries by enhancing antioxidant enzymes and preventing inflammatory cell infiltration.
05:40
Chlorogenic acid attenuates liver apoptosis and inflammation in endoplasmic reticulum stress-induced mice. GreenMedInfo
PMID: Iran J Basic Med Sci. 2023 Apr ;26(4):478-485. PMID: 37009010 Abstract Title: Chlorogenic acid attenuates liver apoptosis and inflammation in endoplasmic reticulum stress-induced mice. Abstract: OBJECTIVES: The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER)results in a state known as "ER stress". It can affect the fate of proteins and play a crucial role in the pathogenesis of several diseases. In this study, we investigated the protective effect of chlorogenic acid (CA) on the inflammation and apoptosis of tunicamycin-induced ER stress in mice.MATERIALS AND METHODS: We categorized mice into six groups: Saline, Vehicle, CA, TM, CA 20-TM, and CA 50-TM. The mice received CA (20 or 50 mg/kg) before intraperitoneal tunicamycin injection. After 72 hr of treatment, serum biochemical analysis, histopathological alterations, protein and/or mRNA levels of steatosis, and inflammatory and apoptotic markers were investigated by ELISA and/or RT-PCR.RESULTS: We found that 20 mg/kg CA decreased mRNA levels of, and. Moreover, CA supplementation prevented TM-induced liver injury through changes in lipid accumulation and lipogenesis markers of steatosis (), and exerted an inhibitory effect on inflammatory (and) and apoptotic markers (caspase 3,,, and), of liver tissue in ER stress mice.CONCLUSION: These data suggest that CA ameliorates hepatic apoptosis and inflammation by reducing NF-B and Caspase 3 as related key factors between inflammation and apoptosis.
05:37
N-acetyl cysteine and chlorogenic acid protection against cisplatin-induced nephrotoxicity. GreenMedInfo
PMID: Pharmaceuticals (Basel). 2023 Feb 22 ;16(3). Epub 2023 Feb 22. PMID: 36986437 Abstract Title: TLR4/Inflammasomes Cross-Talk and Pyroptosis Contribute to N-Acetyl Cysteine and Chlorogenic Acid Protection against Cisplatin-Induced Nephrotoxicity. Abstract: BACKGROUND: Cisplatin (Cp) is an antineoplastic agent with a dose-limiting nephrotoxicity. Cp-induced nephrotoxicity is characterized by the interplay of oxidative stress, inflammation, and apoptosis. Toll-4 receptors (TLR4) and NLPR3 inflammasome are pattern-recognition receptors responsible for activating inflammatory responses and are assigned to play a significant role with gasdermin (GSDMD) in acute kidney injuries. N-acetylcysteine (NAC) and chlorogenic acid (CGA) have documented nephroprotective effects by suppressing oxidative and inflammatory pathways. Therefore, the current study aimed to investigate the contribution of the upregulation of TLR4/inflammasomes/gasdermin signaling to Cp-induced nephrotoxicity and their modulation by NAC or CGA.METHODS: A single injection of Cp (7 mg/kg, i.p.) was given to Wistar rats. Rats received either NAC (250 mg/kg, p.o.) and/or CGA (20 mg/kg, p.o.) one week before and after the Cp injection.RESULTS: Cp-induced acute nephrotoxicity was evident by the increased blood urea nitrogen and serum creatinine and histopathological insults. Additionally, nephrotoxicity was associated with increased lipid peroxidation, reduced antioxidants, and elevated levels of inflammatory markers (NF-B and TNF-) in the kidney tissues. Moreover, Cp upregulated both TLR4/NLPR3/interleukin-1beta (IL-1) and caspase-1/GSDMD-signaling pathways, accompanied by an increased Bax/BCL-2 ratio, indicating an inflammatory-mediated apoptosis. Both NAC and/or CGA significantly corrected these changes.CONCLUSIONS: This study emphasizes that inhibition of TLR4/NLPR3/IL-1/GSDMD might be a novel mechanism of the nephroprotective effects of NAC or CGA against Cp-induced nephrotoxicity in rats.
05:33
Chlorogenic acid restores ovarian functions in mice with letrozole-induced polycystic ovarian syndrome. GreenMedInfo
PMID: Biomedicines. 2023 Mar 14 ;11(3). Epub 2023 Mar 14. PMID: 36979879 Abstract Title: Chlorogenic Acid Restores Ovarian Functions in Mice with Letrozole-Induced Polycystic Ovarian Syndrome Via Modulation of Adiponectin Receptor. Abstract: Around the world, polycystic ovary syndrome (PCOS) is a complex endocrine-metabolic condition that typically affects 6-20% of females. Our study's major goal was to examine how chlorogenic acid (CGA) affected mice with endocrine and metabolic problems brought on by letrozole-induced PCOS. Group I served as the control for 81 days; Group II was given Letrozole (LETZ) orally at a dose of 6 mg/kg bw for 21 days to induce PCOS; Group III was given LETZ (6 mg/kg) for 21 days, followed by treatment with CGA (50 mg/kg bw daily) for 60 days. The study indicated that LETZ-treated mice displayed symptoms of PCOS, such as dyslipidemia, hyperinsulinemia, elevated testosterone, increases in inflammatory markers and malonaldehyde, and a decline in antioxidants (Ar, lhr, fshr, and esr2) in the ovaries. These alterations were affected when the mice were given CGA and were associated with reduced levels of adiponectin. Adiponectin showed interactions with hub genes, namely MLX interacting protein like (MLXIPL), peroxisome proliferator-activated receptor gamma Coactivator 1- alpha (PPARGC1), peroxisome proliferator-activated receptor gamma (Pparg), and adiponectin receptor 1 (Adipor1). Lastly, the gene ontology of adiponectin revealed that adiponectin was highly involved in biological processes. The findings from our research suggest that adiponectin has direct impacts on metabolic and endocrine facets of PCOS.
05:31
Effects of dietary chlorogenic acid on intestinal barrier function and the inflammatory response in broilers during lipopolysaccharide-induced immune stress. GreenMedInfo
PMID: Poult Sci. 2023 May ;102(5):102623. Epub 2023 Mar 4. PMID: 36972676 Abstract Title: Effects of dietary chlorogenic acid on intestinal barrier function and the inflammatory response in broilers during lipopolysaccharide-induced immune stress. Abstract: Immune stress exerts detrimental effects on growth performance and intestinal barrier function during intensive animal production with ensuing serious economic consequences. Chlorogenic acid (CGA) is used widely as a feed additive to improve the growth performance and intestinal health of poultry. However, the effects of dietary CGA supplementation on amelioration of the intestinal barrier impairment caused by immune stress in broilers are unknown. This study investigated the effects of CGA on growth performance, intestinal barrier function, and the inflammatory response in lipopolysaccharide (LPS) mediated immune-stressed broilers. Three hundred and twelve 1-day-old male Arbor Acres broilers were divided randomly into 4 groups with 6 replicates of thirteen broilers. The treatments included: i) saline group: broilers injected with saline and fed with basal diet; ii) LPS group: broilers injected with LPS and fed with basal diet; iii) CGA group: broilers injected with saline and feed supplemented with CGA; and iv) LPS+CGA group: broilers injected with LPS and feed supplemented with CGA. Animals in the LPS and LPS+CGA groups were injected intraperitoneally with an LPS solution prepared with saline from 14 d of age for 7 consecutive days, whereas broilers in the other groups were injected only with saline. LPS induced a decrease in feed intake of broilers during the stress period, but CGA effectively alleviated this decrease. Moreover, CGA inhibited the reduction of villus height and improved the ratio of villus height to crypt depth in the duodenum of broilers 24 and 72 h after LPS injection. In addition, dietary CGA supplementation significantly restored the expression of cation-selective and channel-forming Claudin2 protein 2 h after LPS injection in the ileum. LPS enhanced the expression of tumor necrosis factor-(TNF-) and interleukin-1(IL-1) in the small intestine, but this enhancement was blocked by CGA supplementation. The expression of interleukin-10 (IL-10) increased with LPS injection and CGA promoted the production of IL-10. CGA addition downregulated the expression of intestinal interleukin-6 (IL-6) of broilers under normal rearing conditions. However, CGA supplementation upregulated the expression of IL-6 of broilers 72 h after LPS injection. The data demonstrate that dietary supplementation with CGA alleviates intestinal barrier damage and intestinal inflammation induced by LPS injection during immune stress thereby improving growth performance of broilers.
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05:28
Chlorogenic acid ameliorated non-alcoholic steatohepatitis. GreenMedInfo
PMID: Chem Biol Interact. 2023 May 1 ;376:110461. Epub 2023 Mar 23. PMID: 36965689 Abstract Title: Chlorogenic acid ameliorated non-alcoholic steatohepatitis via alleviating hepatic inflammation initiated by LPS/TLR4/MyD88 signaling pathway. Abstract: Non-alcoholic steatohepatitis (NASH) is a severe pathological stage in non-alcoholic fatty liver disease (NAFLD) and is generally recognized to be induced by chronic inflammation. Natural compound chlorogenic acid (CGA) is well-known for its anti-inflammatory capacity. This study aimed at evaluating the alleviation of CGA on NASH and further exploring its engaged mechanism via focusing on abrogating hepatic inflammation. Our results showed that CGA had a good amelioration on NASH in vivo. CGA alleviated liver oxidative injury by inducing nuclear factor erythroid 2-related factor 2 (Nrf2) activation and reduced liver steatosis via up-regulating peroxisome proliferator-activated receptor-alpha (PPAR). CGA attenuated hepatic inflammation in vivo, but didn't decrease the elevated lipopolysaccharide (LPS) content. CGA blocked the activation of nuclear factor kappa-B (NFB) or inflammasome both in MCDD-fed mice and in LPS-stimulated macrophages. CGA was found to directly bind to myeloid differentiation primary response 88 (MyD88), and thus competitively blocked the interaction between toll-like receptor 4 (TLR4) and MyD88, thereby abrogating hepatic inflammation initiated by LPS-TLR4-MyD88. Moreover, the CGA-provided anti-inflammatory effect was obviously disappeared in macrophages overexpressed MyD88. Hence, CGA has an excellent efficacy in improving NASH. CGA alleviated liver inflammation during NASH progression through blocking LPS-TLR4-MyD88 signaling pathway via directly binding to MyD88.
05:27
Chlorogenic acid may be a viable therapeutic option for bacterial and viral-induced acute respiratory distress syndrome-like pathology. GreenMedInfo
PMID: Clin Sci (Lond). 2023 May 31 ;137(10):785-805. PMID: 36951146 Abstract Title: Targeting TLR4/3 using chlorogenic acid ameliorates LPS+POLY I:C-induced acute respiratory distress syndrome via alleviating oxidative stress-mediated NLRP3/NF-B axis. Abstract: Acute lung injury (ALI) or acute respiratory distress syndrome (ARDS) is a life-threatening condition caused due to significant pulmonary and systemic inflammation. Chlorogenic acid (CGA) has been shown to possess potent antioxidant, anti-inflammatory, and immunoprotective properties. However, the protective effect of CGA on viral and bacterial-induced ALI/ARDS is not yet explored. Hence, the current study is aimed to evaluate the preclinical efficacy of CGA in lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid (POLY I:C)-induced ALI/ARDS models in vitro and in vivo. Human airway epithelial (BEAS-2B) cells exposed to LPS+POLY I:C significantly elevated oxidative stress and inflammatory signaling. Co-treatment with CGA (10 and 50M) prevented inflammation and oxidative stress mediated by TLR4/TLR3 and NLRP3 inflammasome axis. BALB/c mice, when chronically challenged with LPS+POLY I:C showed a significant influx of immune cells, up-regulation of pro-inflammatory cytokines, namely: IL-6, IL-1, and TNF-, and treatment with intranasal CGA (1 and 5 mg/kg) normalized the elevated levels of immune cell infiltration as well as pro-inflammatory cytokines. D-Dimer, the serum marker for intravascular coagulation, was significantly increased in LPS+ POLY I:C challenged animals which was reduced with CGA treatment. Further, CGA treatment also has a beneficial effect on the lung and heart, as shown by improving lung physiological and cardiac functional parameters accompanied by the elevated antioxidant response and simultaneous reduction in tissue damage caused by LPS+POLY I:C co-infection. In summary, these comprehensive, in vitro and in vivo studies suggest that CGA may be a viable therapeutic option for bacterial and viral-induced ALI-ARDS-like pathology.
05:24
A chlorogenic acid functional strategy of anti-inflammation, anti-coagulation and promoted endothelial proliferation for bioprosthetic artificial heart valves. GreenMedInfo
PMID: J Mater Chem B. 2023 Mar 22 ;11(12):2663-2673. Epub 2023 Mar 22. PMID: 36883900 Abstract Title: A chlorogenic acid functional strategy of anti-inflammation, anti-coagulation and promoted endothelial proliferation for bioprosthetic artificial heart valves. Abstract: Heart valve replacement has become an optimal choice for the treatment of severe heart valve disease. At present, most commercial bioprosthetic heart valves (BHVs) are made from porcine pericardium or bovine pericardium treated with glutaraldehyde. Nevertheless, due to the toxicity of residual aldehyde groups left after glutaraldehyde cross-linking, these commercial BHVs exhibit poor biocompatibility, calcification, risk of coagulation and endothelialization difficulty, which greatly affects the durability of the BHVs and shortens their service life. In this work, based on a chlorogenic acid functional anti-inflammation, anti-coagulation and endothelialization strategy and dual-functional non-glutaraldehyde cross-linking reagent OX-CO, a kind of functional BHV material OX-CA-PP has been developed from OX-CO cross-linked porcine pericardium (OX-CO-PP) followed by the convenient modification of chlorogenic acid through a reactive oxygen species (ROS) sensitive borate ester bond. The functionalization of chlorogenic acid can reduce the risk of valve leaf thrombosis and promote endothelial cell proliferation, which is beneficial to the formation of a long-term interface with good blood compatibility. Meanwhile, such a ROS responsive behavior can trigger intelligent release of chlorogenic acid on-demand to achieve the inhibition of acute inflammation at the early stage of implantation. Theandexperimental results show that the functional BHV material OX-CA-PP exhibits superior anti-inflammation, improved anti-coagulation, minimal calcification and promoted proliferation of endothelial cells, showing that this non-glutaraldehyde functional strategy has great potential for the application of BHVs and providing a promising reference for other implanted biomaterials.
05:22
Chlorogenic acid rich in coffee pulp extract suppresses inflammatory status. GreenMedInfo
PMID: Heliyon. 2023 Mar ;9(3):e13917. Epub 2023 Feb 18. PMID: 36873494 Abstract Title: Chlorogenic acid rich in coffee pulp extract suppresses inflammatory status by inhibiting the p38, MAPK, and NF-B pathways. Abstract: Coffee pulp (CP) is a coffee byproduct that contains various active ingredients, namely, chlorogenic acid (CGA) and caffeine. These active compounds show several benefits, including antihyperlipidemia, antioxidants, and anti-inflammation. However, the anti-inflammatory properties of Coffea pulp extract (CPE) are unknown. This work determined the impact of CPE on lipopolysaccharide (LPS)-activated murine macrophage cells and the molecular mechanism behind this action. RAW 264.7 cells were exposed to varying doses of CPE with or without LPS. Inflammatory markers and their mechanism were studied. CPE therapy has been shown to suppress the synthesis of inflammatory cytokines and mediators, namely, tumor necrosis factor-alpha (TNF-), interleukin-6 (IL-6), IL-1, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and nitric oxide (NO), as well as prostaglandin E2 (PGE). Finally, CPE inactivated the nuclear factor-kappa B (NF-B) and MAPK signaling pathways. Consequently, CPE might be used as a nutraceutical to treat inflammation and its related disorders.
05:19
E-cigarette liquids and aldehyde flavoring agents inhibit CYP2A6 activity in lung epithelial cells. GreenMedInfo
PMID: ACS Omega. 2023 Mar 28 ;8(12):11261-11266. Epub 2023 Mar 13. PMID: 37008141 Abstract Title: E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells. Abstract: Certain e-liquids and aromatic aldehyde flavoring agents were previously identified as inhibitors of microsomal recombinant CYP2A6, the primary nicotine-metabolizing enzyme. However, due to their reactive nature, aldehydes may react with cellular components before reaching CYP2A6 in the endoplasmic reticulum. To determine whether e-liquid flavoring agents inhibited CYP2A6 in a cellular system, we investigated their effects on CYP2A6 using BEAS-2B cells transduced to overexpress CYP2A6. We demonstrated that two e-liquids and three aldehyde flavoring agents (cinnamaldehyde, benzaldehyde, and ethyl vanillin) exhibited dose-dependent inhibition of cellular CYP2A6.
05:17
Aerosolized nicotine from e-cigarettes alters gene expression, increases lung protein permeability, and impairs viral clearance in murine influenza infection. GreenMedInfo
PMID: Front Immunol. 2023 ;14:1076772. Epub 2023 Mar 14. PMID: 36999019 Abstract Title: Aerosolized nicotine from e-cigarettes alters gene expression, increases lung protein permeability, and impairs viral clearance in murine influenza infection. Abstract: E-cigarette use has rapidly increased as an alternative means of nicotine delivery by heated aerosolization. Recent studies demonstrate nicotine-containing e-cigarette aerosols can have immunosuppressive and pro-inflammatory effects, but it remains unclear how e-cigarettes and the constituents of e-liquids may impact acute lung injury and the development of acute respiratory distress syndrome caused by viral pneumonia. Therefore, in these studies, mice were exposed one hour per day over nine consecutive days to aerosol generated by the clinically-relevant tank-style Aspire Nautilus aerosolizing e-liquid containing a mixture of vegetable glycerin and propylene glycol (VG/PG) with or without nicotine. Exposure to the nicotine-containing aerosol resulted in clinically-relevant levels of plasma cotinine, a nicotine-derived metabolite, and an increase in the pro-inflammatory cytokines IL-17A, CXCL1, and MCP-1 in the distal airspaces. Following the e-cigarette exposure, mice were intranasally inoculated with influenza A virus (H1N1 PR8 strain). Exposure to aerosols generated from VG/PG with and without nicotine caused greater influenza-induced production in the distal airspaces of the pro-inflammatory cytokines IFN-, TNF, IL-1, IL-6, IL-17A, and MCP-1 at 7 days post inoculation (dpi). Compared to the aerosolized carrier VG/PG, in mice exposed to aerosolized nicotine there was a significantly lower amount of Mucin 5 subtype AC (MUC5AC) in the distal airspaces and significantly higher lung permeability to protein and viral load in lungs at 7 dpi with influenza. Additionally, nicotine caused relative downregulation of genes associated with ciliary function and fluid clearance and an increased expression of pro-inflammatory pathways at 7 dpi. These results show that (1) the e-liquid carrier VG/PG increases the pro-inflammatory immune responses to viral pneumonia and that (2) nicotine in an e-cigarette aerosol alters the transcriptomic response to pathogens, blunts host defense mechanisms, increases lung barrier permeability, and reduces viral clearance during influenza infection. In conclusion, acute exposure to aerosolized nicotine can impair clearance of viral infection and exacerbate lung injury, findings that have implications for the regulation of e-cigarette products.
05:16
Vaping should be stopped in the perioperative period to decrease the incidence of wound healing complications. GreenMedInfo
PMID: Surgery. 2023 Jun ;173(6):1452-1462. Epub 2023 Mar 29. PMID: 36997424 Abstract Title: The implications of vaping on surgical wound healing: A systematic review. Abstract: BACKGROUND: E-cigarette use is rapidly growing, and little is known about the postoperative complications. Cigarette smoking has been well-established to be associated with delayed wound healing and increased complications in surgical patients. Due to the intricate and harmonious nature of the wound-healing process, vaping may impair tissue regeneration, posing a risk for patients undergoing surgery. This systematic review aimed to review the evidence on the implications of vaping on wound healing.METHODS: A systematic search of PubMed and Scopus databases was conducted on October 2022 per Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The following keywords were used to conduct the search: vaping, vape, e-cigarettes, electronic cigarettes, wound healing, tissue regeneration, postoperative complications, wound infection, and blood flow.RESULTS: Of 5,265 screened articles, only 37 were eligible for qualitative synthesis. A total of 18 articles studied the effect of e-cigarettes on human volunteers, 14 investigated e-cigarette extract on human cell lines, and 5 used animal rat models.CONCLUSION: Despite limited objective data, the recommendation is that e-cigarettes be treated as tobacco cigarettes; hence, vaping should be stopped in the perioperative period to decrease the incidence of wound healing complications. Clinical trials are required to understand the health hazards of e-cigarettes further and maximize patient safety and clinical outcomes.
05:13
Flavored E-cigarette product aerosols induce transformation of human bronchial epithelial cells. GreenMedInfo
PMID: Lung Cancer. 2023 May ;179:107180. Epub 2023 Mar 22. PMID: 36989612 Abstract Title: Flavored E-cigarette product aerosols induce transformation of human bronchial epithelial cells. Abstract: OBJECTIVES: E-cigarettes are the most commonly used nicotine containing products among youth. In vitro studies support the potential for e-cigarettes to cause cellular stress in vivo; however, there have been no studies to address whether exposure to e-liquid aerosols can induce cell transformation, a process strongly associated with pre-malignancy. We examined whether weekly exposure of human bronchial epithelial cell (HBEC) lines to e-cigarette aerosols would induce transformation and concomitant changes in gene expression and promoter hypermethylation.MATERIALS AND METHODS: An aerosol delivery system exposed three HBEC lines to unflavored e-liquid with 1.2% nicotine, 3 flavored products with nicotine, or the Kentucky reference cigarette once a week for 12 weeks. Colony formation in soft agar, RNA-sequencing, and the EPIC Beadchip were used to evaluate transformation, genome-wide expression and methylation changes.RESULTS: Jamestown e-liquid aerosol induced transformation of HBEC2 and HBEC26, while unflavored and Blue Pucker transformed HBEC26. Cigarette smoke aerosol transformed HBEC4 and HBEC26 at efficiencies up to 3-fold greater than e-liquids. Transformed clones exhibited extensive reprogramming of the transcriptome with common and distinct gene expression changes observed between the cigarette and e-liquids. Transformation by e-liquids induced alterations in canonical pathways implicated in lung cancer that included axonal guidance and NRF2. Gene methylation, while prominent in cigarette-induced transformed clones, also affected hundreds of genes in HBEC2 transformed by Jamestown. Many genes with altered expression or epigenetic-mediated silencing were also affected in lung tumors from smokers.CONCLUSIONS: These studies show that exposure to e-liquid aerosols can induce a pre-malignant phenotype in lung epithelial cells. While the Food and Drug Administration banned the sale of flavored cartridge-based electric cigarettes, consumers switched to using flavored products through other devices. Our findings clearly support expanding studies to evaluate transformation potency for the major categories of e-liquid flavors to better inform risk from these complex mixtures.
05:12
Electronic cigarette exposure increases the severity of influenza a virus infection. GreenMedInfo
PMID: Int J Mol Sci. 2023 Feb 21 ;24(5). Epub 2023 Feb 21. PMID: 36901724 Abstract Title: Electronic Cigarette Exposure Increases the Severity of Influenza a Virus Infection via TRAIL Dysregulation in Human Precision-Cut Lung Slices. Abstract: The use of electronic nicotine dispensing systems (ENDS), also known as electronic cigarettes (ECs), is common among adolescents and young adults with limited knowledge about the detrimental effects on lung health such as respiratory viral infections and underlying mechanisms. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a protein of the TNF family involved in cell apoptosis, is upregulated in COPD patients and during influenza A virus (IAV) infections, but its role in viral infection during EC exposures remains unclear. This study was aimed to investigate the effect of ECs on viral infection and TRAIL release in a human lung precision-cut lung slices (PCLS) model, and the role of TRAIL in regulating IAV infection. PCLS prepared from lungs of nonsmoker healthy human donors were exposed to EC juice (E-juice) and IAV for up to 3 days during which viral load, TRAIL, lactate dehydrogenase (LDH), and TNF-in the tissue and supernatants were determined. TRAIL neutralizing antibody and recombinant TRAIL were utilized to determine the contribution of TRAIL to viral infection during EC exposures. E-juice increased viral load, TRAIL, TNF-release and cytotoxicity in IAV-infected PCLS. TRAIL neutralizing antibody increased tissue viral load but reduced viral release into supernatants. Conversely, recombinant TRAIL decreased tissue viral load but increased viral release into supernatants. Further, recombinant TRAIL enhanced the expression of interferon-and interferon-induced by E-juice exposure in IAV-infected PCLS. Our results suggest that EC exposure in human distal lungs amplifies viral infection and TRAIL release, and that TRAIL may serve as a mechanism to regulate viral infection. Appropriate levels of TRAIL may be important to control IAV infection in EC users.
05:10
Effect of supplemental antioxidant-based therapy on the oxidative stress level in COVID-19 patients. GreenMedInfo
PMID: Pril (Makedon Akad Nauk Umet Odd Med Nauki). 2023 Mar 1 ;44(1):7-16. Epub 2023 Mar 29. PMID: 36987766 Abstract Title: Effect of Supplemental Antioxidant-Based Therapy on the Oxidative Stress Level in COVID-19 Patients. Abstract: : COVID-19 is a disease in several stages starting with virus replication to dysregulation in immune system response, organ failure and recovery/death. Our aim was to determine the effect of Ganoderma lucidum, lycopene, sulforaphane, royal jelly and resveratrol extract on markers of oxidative stress, inflammation, routine laboratory analyses and duration of symptoms in COVID-19 patients.: The oxidative stress parameters and interleukines 6 and 8 (IL-6, IL-8), tumor necrosis factor alpha (TNF-) were determined in order to estimate the antioxidant and the anti-inflammatory effect of the product using a spectrophotometric and a magnetic bead-based multiplex assay in serum of 30 patients with mild form of COVID-19.: Statistically significant differences were obtained for all investigated parameters between the treated patients and the control group. Moreover, significant differences were observed for leukocytes, neutrophil to leukocyte ratio and iron. The average duration of the symptoms was 9.40.487 days versus 13.10.483 days in the treatment and the control group, respectively (p=0.0003).: Our results demonstrated the promising effect of Ge132+Naturalon reducing the oxidative stress and the IL-6, IL-8 and TNF-levels, and symptoms duration in COVID-19 patients. The evidence presented herein suggest that the combination of Ganoderma lucidum extract, lycopene, sulforaphane, royal jelly and resveratrol could be used as a potent an adjuvant therapy in diseases accompanied by increased oxidative stress and inflammation.
05:07
Ganoderma lucidum polysaccharides attenuates pressure-overload-induced pathological cardiac hypertrophy. GreenMedInfo
PMID: Front Pharmacol. 2023 ;14:1127123. Epub 2023 Mar 23. PMID: 37033616 Abstract Title: polysaccharides attenuates pressure-overload-induced pathological cardiac hypertrophy. Abstract: Pathological cardiac hypertrophy is an important risk factor for cardiovascular disease. However, drug therapies that can reverse the maladaptive process and restore heart function are limited.polysaccharides (GLPs) are one of the main active components of(), and they have various pharmacological effects. GLPs have been used as Chinese medicine prescriptions for clinical treatment. In this study, cardiac hypertrophy was induced by transverse aortic constriction (TAC) in mice. We found that GLPs ameliorate Ang II-induced cardiomyocyte hypertrophyand attenuate pressure overload-induced cardiac hypertrophy. Further research indicated that GLPs attenuated the mRNA levels of hypertrophic and fibrotic markers to inhibit cardiac hypertrophy through the PPAR/PGC-1pathway. Overall, these results indicate that GLPs inhibit cardiac hypertrophy through downregulating key genes for hypertrophy and fibrosis and attenuate pressure overload-induced pathological cardiac hypertrophy by activating PPAR. This study provides important theoretical support for the potential of using GLPs to treat pathological myocardial hypertrophy and heart failure.
05:05
Potential anti-rheumatoid arthritis activities and mechanisms of Ganoderma lucidum polysaccharides. GreenMedInfo
PMID: Molecules. 2023 Mar 8 ;28(6). Epub 2023 Mar 8. PMID: 36985456 Abstract Title: Potential Anti-Rheumatoid Arthritis Activities and Mechanisms ofPolysaccharides. Abstract: Rheumatoid arthritis (RA) is a chronic and autoimmune disease characterized by inflammation, autoimmune dysfunction, and cartilage and bone destruction. In this review, we summarized the available reports on the protective effects ofpolysaccharides (GLP) on RA in terms of anti-inflammatory, immunomodulatory, anti-angiogenic and osteoprotective effects. Firstly, GLP inhibits RA synovial fibroblast (RASF) proliferation and migration, modulates pro- and anti-inflammatory cytokines and reduces synovial inflammation. Secondly, GLP regulates the proliferation and differentiation of antigen-presenting cells such as dendritic cells, inhibits phagocytosis by mononuclear macrophages and nature killer (NK) cells and regulates the ratio of M1, M2 and related inflammatory cytokines. In addition, GLP produced activities in balancing humoral and cellular immunity, such as regulating immunoglobulin production, modulating T and B lymphocyte proliferative responses and cytokine release, exhibiting immunomodulatory effects. Thirdly, GLP inhibits angiogenesis through the direct inhibition of vascular endothelial cell proliferation and induction of cell death and the indirect inhibition of vascular endothelial growth factor (VEGF) production in the cells. Finally, GLP can inhibit the production of matrix metalloproteinases and promote osteoblast formation, exerting protective effects on bone and articular cartilage. It is suggested that GLP may be a promising agent for the treatment of RA.
05:03
Reishi and Lion's Mane mushroom powder can be used for the fortification of semolina pasta. GreenMedInfo
PMID: Antioxidants (Basel). 2023 Mar 17 ;12(3). Epub 2023 Mar 17. PMID: 36978986 Abstract Title: The Addition of Reishi and Lion's Mane Mushroom Powder to Pasta Influences the Content of Bioactive Compounds and the Antioxidant, Potential Anti-Inflammatory, and Anticancer Properties of Pasta. Abstract: The influence of a 2.5% and 5% addition of dried Reishi and Lion's Mane mushrooms on the content of bioactive compounds and some pro-health properties of pasta was studied. In samples subjected to gastrointestinal digestion, the content of phenolic compounds and the antioxidant, potential anti-inflammatory, and antiproliferative properties were significantly higher. The qualitative-quantitative analysis of phenolic compounds performed using the LC-MS/MS technique indicated that the Reishi-enriched pasta was characterized by a higher content of syringic (R2.5 sample), while pasta supplemented with Lion' Mane had a higher content of vanillin in relation to the control pasta. In the case of ethanolic extracts, samples supplemented with the Reishi mushrooms (R5 sample) were characterized by higher ABTS antiradical properties and a reducing power while the sample supplemented with Lion's Mane (L5 sample) had a higher ability to inhibit lipoxygenase in relation to the control sample. In conclusion, the results suggest that Reishi and Lion's Mane mushroom powder can be used for the fortification of semolina pasta, conferring slightly healthier characteristics of the product.
05:01
Neuroprotective effects of erinacine A on an experimental model of traumatic optic neuropathy. GreenMedInfo
PMID: Int J Mol Sci. 2023 Jan 12 ;24(2). Epub 2023 Jan 12. PMID: 36675019 Abstract Title: Neuroprotective Effects of Erinacine A on an Experimental Model of Traumatic Optic Neuropathy. Abstract: Erinacine A (EA), a natural neuroprotectant, is isolated from a Chinese herbal medicine, Hericium erinaceus. The aim of this study was to investigate the neuroprotective effects of EA in a rat model of traumatic optic neuropathy. The optic nerves (ONs) of adult male Wistar rats were crushed using a standardized method and divided into three experimental groups: phosphate-buffered saline (PBS control)-treated group, standard EA dose-treated group (2.64 mg/kg in 0.5 mL of PBS), and double EA dose-treated group (5.28 mg/kg in 0.5 mL of PBS). After ON crush, each group was fed orally every day for 14 days before being euthanized. The visual function, retinal ganglion cell (RGC) density, and RGC apoptosis were determined using flash visual-evoked potentials (fVEP) analysis, retrograde Fluoro-Gold labelling, and TdT-dUTP nick end-labelling (TUNEL) assay, respectively. Macrophage infiltration of ON was detected by immunostaining (immunohistochemistry) for ED1. The protein levels of phosphor-receptor-interacting serine/threonine-protein kinase1 (pRIP1), caspase 8 (Cas8), cleaved caspase 3 (cCas3), tumour necrosis factor (TNF)-, tumour necrosis factor receptor1 (TNFR1), interleukin (IL)-1, inducible nitric oxide synthase (iNOS), nuclear factor erythroid 2-related factor 2 (Nrf2), haem oxygenase-1 (HO-1), and superoxide dismutase 1 (SOD1) were evaluated by Western blotting. When comparing the standard EA dose-treated group and the double EA dose-treated group with the PBS-treated group, fVEP analysis showed that the amplitudes of P1N2 in the standard EA dose group and the double EA dose-treated group were 1.8 and 2.4-fold, respectively, higher than that in the PBS-treated group (p
04:57
Potential antidepressant effects of a dietary supplement from the chlorella and lion's mane mushroom complex. GreenMedInfo
PMID: Front Nutr. 2022 ;9:977287. Epub 2022 Sep 2. PMID: 36118772 Abstract Title: Potential antidepressant effects of a dietary supplement from the chlorella and lion's mane mushroom complex in aged SAMP8 mice. Abstract: Since the 1990s, the prevalence of mental illnesses, such as depression, has been increasing annually and has become a major burden on society. Due to the many side effects of antidepressant drugs, the development of a complementary therapy from natural materials is an urgent need. Therefore, this study used a complex extract of chlorella and lion's mane mushroom and evaluated its antidepressant effects. Six-month-old male senescence-accelerated mice prone-8 (SAMP8) were divided into positive control; negative control; and low, medium, and high-dose groups. All groups were treated with corticosterone (CORT) at 40 mg/Kg/day for 21- days to induce depression in the animals, and the effects of different test substances on animal behavior was observed. The positive control group was intraperitoneally injected with a tricyclic antidepressant (Fluoxetine, as tricyclic antidepressant), the control group was given ddHO, and the test substance groups were administered test samples once daily for 21 days. The open field test (OFT) and forced swimming test (FST) were applied for behavior analyses of depression animal models. The OFT results showed that the mice in the positive control and the medium-, and high-dose groups demonstrated a significantly prolonged duration in the central area and a significantly increased travel distance. In the FST, the positive control and the medium, and high-dose groups displayed significantly reduced immobility times relative to the control group. The blood analysis results showed significant decreases in triglyceride and blood urea nitrogen levels relative to the positive control and the medium- and high-dose groups. Notably, in the positive control and the medium- and high-dose groups, brain-derived neurotrophic factor (BDNF) increase by more than in the control group. In summary, medium and high dose of extract of chlorella and lion's mane mushroom could improve depression behavior in animals and have the potential to be antidepressant health care products.
04:54
The effects of Chlorella vulgaris on cardiovascular risk factors. GreenMedInfo
PMID: Biomed Pharmacother. 2023 Jun ;162:114624. Epub 2023 Apr 3. PMID: 37018990 Abstract Title: The effects of Chlorella vulgaris on cardiovascular risk factors: A comprehensive review on putative molecular mechanisms. Abstract: High incidence rate of cardiovascular disease (CVD) make this condition as an important public health concern. The use of natural products in treating this chronic condition has increased in recent years one of which is the single-celled green alga Chlorella. Chlorella vulgaris (CV) has been studied for its potential benefits to human health due to its biological and pharmacological features. CV contains a variety of macro and micronutrients, including proteins, omega-3, polysaccharides, vitamins, and minerals. Some studies have indicated that taking CV as a dietary supplement can help reduce inflammation and oxidative stress. In some studies, cardiovascular risk factors that are based on hematological indices did not show these benefits, and no molecular mechanisms have been identified. This comprehensive review summarized the research on the cardio-protective benefits of chlorella supplementation and the underlying molecular processes.
04:52
Royal jelly and Chlorella vulgaris mitigate gibberellic acid-induced cytogenotoxicity and hepatotoxicity. GreenMedInfo
PMID: Foods. 2023 Mar 13 ;12(6). Epub 2023 Mar 13. PMID: 36981150 Abstract Title: Royal Jelly andMitigate Gibberellic Acid-Induced Cytogenotoxicity and Hepatotoxicity in Rats via Modulation of the PPAR/AP-1 Signaling Pathway and Suppression of Oxidative Stress and Inflammation. Abstract: Gibberellic acid (GA3) is a well-known plant growth regulator used in several countries, but its widespread use has negative effects on both animal and human health. The current study assesses the protective effect of royal jelly (RJ) and(CV) on the genotoxicity and hepatic injury induced by GA3 in rats. Daily oral administration of 55 mg/kg GA3 to rats for 6 constitutive weeks induced biochemical and histopathological changes in the liver via oxidative stress and inflammation. Co-administration of 300 mg/kg RJ or 500 mg/kg CV with GA3 considerably ameliorated the serum levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase),GT (gamma-glutamyl transferase), total bilirubin, and albumin. Lowered malondialdehyde, tumor necrosis factor(TNF-), and nuclear factorB (NF-B) levels along with elevated SOD (superoxide dismutase), CAT (catalase), and GPx (glutathione peroxidase) enzyme activities indicated the antioxidant and anti-inflammatory properties of both RJ and CV. Also, they improved the histological structure and reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions along with up-regulating peroxisome proliferator activated receptor(PPAR) and down-regulating activator protein 1 (AP-1) gene expression. Additionally, chromosomal abnormalities and mitotic index were nearly normalized after treatment with RJ and CV. In conclusion, RJ and CV can protect against GA3-induced genotoxicity and liver toxicity by diminishing oxidative stress and inflammation, and modulating the PPAR/AP-1 signaling pathway.
04:49
Chlorella sp.-ameliorated undesirable microenvironment promotes diabetic wound healing. GreenMedInfo
PMID: Acta Pharm Sin B. 2023 Jan ;13(1):410-424. Epub 2022 Jun 23. PMID: 36815029 Abstract Title: sp.-ameliorated undesirable microenvironment promotes diabetic wound healing. Abstract: Chronic diabetic wound remains a critical challenge suffering from the complicated negative microenvironments, such as high-glucose, excessive reactive oxygen species (ROS), hypoxia and malnutrition. Unfortunately, few strategies have been developed to ameliorate the multiple microenvironments simultaneously. In this study,sp. (Chlorella) hydrogels were prepared against diabetic wounds.experiments demonstrated that living Chlorella could produce dissolved oxygen by photosynthesis, actively consume glucose and deplete ROS with the inherent antioxidants, during the daytime. At night, Chlorella was inactivatedby chlorine dioxide with human-body harmless concentration to utilize its abundant contents. It was verifiedthat the inactivated-Chlorella could supply nutrition, relieve inflammation and terminate the oxygen-consumption of Chlorella-respiration. The advantages of living Chlorella and its contents were integrated ingeniously. The abovementioned functions were proven to accelerate cell proliferation, migration and angiogenesis. Then, streptozotocin-induced diabetic mice were employed for further validation. Theoutcomes confirmed that Chlorella could ameliorate the undesirable microenvironments, including hypoxia, high-glucose, excessive-ROS and chronic inflammation, thereby synergistically promoting tissue regeneration. Given the results above, Chlorella is considered as a tailor-made therapeutic strategy for diabetic wound healing.
04:47
Identification of berberine as a potential therapeutic strategy for kidney clear cell carcinoma and COVID-19 based on analysis of large-scale datasets. GreenMedInfo
PMID: Front Immunol. 2023 ;14:1038651. Epub 2023 Mar 23. PMID: 37033923 Abstract Title: Identification of berberine as a potential therapeutic strategy for kidney clear cell carcinoma and COVID-19 based on analysis of large-scale datasets. Abstract: BACKGROUND: Regarding the global coronavirus disease 2019 (COVID)-19 pandemic, kidney clear cell carcinoma (KIRC) has acquired a higher infection probability and may induce fatal complications and death following COVID-19 infection. However, effective treatment strategies remain unavailable. Berberine exhibits significant antiviral and antitumour effects. Thus, this study aimed to provide a promising and reliable therapeutic strategy for clinical decision-making by exploring the therapeutic mechanism of berberine against KIRC/COVID-19.METHODS: Based on large-scale data analysis, the target genes, clinical risk, and immune and pharmacological mechanisms of berberine against KIRC/COVID-19 were systematically investigated.RESULTS: In total, 1,038 and 12,992 differentially expressed genes (DEGs) of COVID-19 and KIRC, respectively, were verified from Gene Expression Omnibus and The Cancer Genome Atlas databases, respectively, and 489 berberine target genes were obtained from official websites. After intersecting, 26 genes were considered potential berberine therapeutic targets for KIRC/COVID-19. Berberine mechanism of action against KIRC/COVID-19 was revealed by protein-protein interaction, gene ontology, and Kyoto Encyclopedia of Genes and Genomes with terms including protein interaction, cell proliferation, viral carcinogenesis, and the PI3K/Akt signalling pathway. In COVID-19 patients, ACOX1, LRRK2, MMP8, SLC1A3, CPT1A, H2AC11, H4C8, and SLC1A3 were closely related to disease severity, and the general survival of KIRC patients was closely related to ACOX1, APP, CPT1A, PLK1, and TYMS. Additionally, the risk signature accurately and sensitively depicted the overall survival and patient survival status for KIRC. Numerous neutrophils were enriched in the immune system of COVID-19 patients, and the lives of KIRC patients were endangered due to significant immune cell infiltration. Molecular docking studies indicated that berberine binds strongly to target proteins.CONCLUSION: This study demonstrated berberine as a potential treatment option in pharmacological, immunological, and clinical practice. Moreover, its therapeutic effects may provide potential and reliable treatment options for patients with KIRC/COVID-19.
04:45
Berberine attenuates epithelial mesenchymal transition in bleomycin-induced pulmonary fibrosis. GreenMedInfo
PMID: Life Sci. 2023 Jun 1 ;322:121665. Epub 2023 Apr 5. PMID: 37028546 Abstract Title: Berberine attenuates epithelial mesenchymal transition in bleomycin-induced pulmonary fibrosis in mice via activating AR and mitigating the SDF-1/CXCR4 signaling. Abstract: AIMS: Berberine is endowed with anti-oxidant, anti-inflammatory and anti-fibrotic effects. This study explored the role of adenosine Areceptor (AR) activation and SDF-1/CXCR4 signaling suppression in the protective effects of berberine in bleomycin-induced pulmonary fibrosis in mice.MAIN METHODS: Pulmonary fibrosis was generated in mice by injecting bleomycin (40 U/kg, i.p.) on days 0, 3, 7, 10 and 14. Mice were treated with berberine (5 mg/kg, i.p.) from day 15 to day 28.KEY FINDINGS: Severe lung fibrosis and increased collagen content were observed in the bleomycin-challenged mice. Pulmonary AR downregulation was documented in bleomycin-induced pulmonary fibrosis animals and was accompanied by enhanced expression of SDF-1/CXCR4. Moreover, TGF-1elevation and pSmad2/3 overexpression were reported in parallel with enhanced epithelial mesenchymal transition (EMT) markers expression, vimentin and-SMA. Besides, bleomycin significantly elevated the inflammatory and pro-fibrogenic mediator NF-B p65, TNF-and IL-6. Furthermore, bleomycin administration induced oxidative stress as depicted by decreased Nrf2, SOD, GSH and catalase levels. Interestingly, berberine administration markedly ameliorated the fibrotic changes in lungs by modulating the purinergic system through the inhibition of AR downregulation, mitigating EMT and effectively suppressing inflammation and oxidative stress. Strikingly, AR blockade by SCH 58261, impeded the pulmonary protective effect of berberine.SIGNIFICANCE: These findings indicated that berberine could attenuate the pathological processes of bleomycin-induced pulmonary fibrosis at least partially via upregulating AR and mitigating the SDF-1/CXCR4 related pathway, suggesting AR as a potential therapeutic target for the management of pulmonary fibrosis.
04:43
Berberine is a suppressor of hedgehog signaling cascade in colorectal cancer. GreenMedInfo
PMID: Phytomedicine. 2023 Jun ;114:154792. Epub 2023 Mar 30. PMID: 37028248 Abstract Title: Berberine is a suppressor of Hedgehog signaling cascade in colorectal cancer. Abstract: BACKGROUND: Colorectal cancer (CRC) is a malignant affliction that burdens people globally. Overactivated Hedgehog signal is highly implicated in CRC pathogenesis. Phytochemical berberine exerts strong potency on CRC, with molecular mechanism elusive.PURPOSE: We sought to study berberine's anti-CRC action and explore its underlying mechanism based on Hedgehog signaling cascade.METHODS: In CRC HCT116 cells and SW480 cells treated with berberine, the proliferation, migration, invasion, clonogenesis, apoptosis and cell cycle were measured, with determination of Hedgehog signaling pathway activity. Following establishment of mouse model of HCT116 xenograft tumor, the efficacies of berberine on carcinogenesis, pathological manifestation and malignant phenotypes of CRC were examined, with analysis of Hedgehog signaling axis in HCT116 xenograft tumor tissues. Additionally, toxicological study of berberine was conducted on zebrafish.RESULTS: Berberine was discovered to suppress the proliferation, migration, invasion and clonogenesis of HCT116 cells and SW480 cells. Furthermore, berberine caused cell apoptosis and blockaded cell cycle at phase G/Gin CRC cells, with dampened Hedgehog signaling cascade. In HCT116 xenograft tumor of nude mice, berberine inhibited tumor growth, alleviated pathological score, and promoted apoptosis and cell cycle arrest in tumor tissues, through constraining Hedgehog signaling. The toxicological study of berberine on zebrafish indicated that berberine incurred damage to the liver and heart of zebrafish at high dosage and prolonged administration.CONCLUSIONS: Taken together, berberine may inhibit the malignant phenotypes of CRC through diminishing Hedgehog signaling cascade. However, the potential adverse reactions should be taken into account upon abuse of berberine.
04:37
Berberine ameliorates the neurological dysfunction of the gastric fundus. GreenMedInfo
PMID: Saudi Pharm J. 2023 Mar ;31(3):433-443. Epub 2023 Feb 2. PMID: 37026044 Abstract Title: Berberine ameliorates the neurological dysfunction of the gastric fundus by promoting calcium channels dependent release of ACh in STZ-induced diabetic rats. Abstract: BACKGROUND: It has been reported diabetic gastroparesis is related to diabetic autonomic neuropathy of the gastrointestinal tract, and berberine (BBR) could ameliorate diabetic central and peripheral neuropathy. However, the influence of BBR on the function and motility of the gastric fundus nerve is unclear.METHODS: A diabetic rat model was constructed, and HE staining was used to observe the morphological changes in the gastric fundus. The changes in cholinergic and nitrogen-related neurochemical indexes and the effects of BBR on them were measured using Elisa. The effects of BBR on the neural function and motility of gastric fundus were investigated by electric field stimulation (EFS) induced neurogenic response in vitro.RESULTS: In the early stage of STZ-induced diabetic rats, the contractile response of gastric fundus induced by EFS was disorder, disturbance of contraction amplitude, and the cell bodies of neurons in the myenteric plexus of gastric fundus presented vacuolar lesions. Administration with BBR could improve the above symptoms. BBR further enhanced the contraction response in the presence of a NOS inhibitor or the case of inhibitory neurotransmitters removal. Interestingly, the activity of ACh could affect NO release directly and the enhancement of BBR on contractile response was canceled by calcium channel blockers completely.CONCLUSIONS: In the early stage of STZ-induced diabetic rats, the neurogenic contractile response disorder of the gastric fundus is mainly related to cholinergic and nitrergic nerve dysfunction. BBR promotes the release of ACh mainly by affecting the calcium channel to improve the neurological dysfunction of the gastric fundus.
04:34
Berberine rescues D-ribose-induced Alzheimer's pathology. GreenMedInfo
PMID: Int J Mol Sci. 2023 Mar 20 ;24(6). Epub 2023 Mar 20. PMID: 36982968 Abstract Title: Berberine Rescues D-Ribose-Induced Alzheimer's Pathology via Promoting Mitophagy. Abstract: Mitochondrial dysfunction is considered an early event of Alzheimer disease (AD). D-ribose is a natural monosaccharide that exists in cells, especially in mitochondria, and can lead to cognitive dysfunction. However, the reason for this is unclear. Berberine (BBR) is an isoquinoline alkaloid that can target mitochondria and has great prospect in the treatment of AD. The methylation of PINK1 reinforces the burden of Alzheimer's pathology. This study explores the role of BBR and D-ribose in the mitophagy and cognitive function of AD related to DNA methylation.mice andcells were treated with D-ribose, BBR, and mitophagy inhibitor Mdivi-1 to observe their effects on mitochondrial morphology, mitophagy, neuron histology, AD pathology, animal behavior, and PINK1 methylation. The results showed that D-ribose induced mitochondrial dysfunction, mitophagy damage, and cognitive impairment. However, BBR inhibition of PINK1 promoter methylation can reverse the above effects caused by D-ribose, improve mitochondrial function, and restore mitophagy through the PINK1-Parkin pathway, thus reducing cognitive deficits and the burden of AD pathology. This experiment puts a new light on the mechanism of action of D-ribose in cognitive impairment and reveals new insights in the use of BBR for AD treatment.
04:14
The Government Has a Moral Duty to Help Those Harmed by Prescription Drugs Mad In America
From The BMJ: This week, BBC1s Panorama drew sharp attention to the problem of prescribed drug dependence and withdrawal in the United Kingdom, providing insight into an iatrogenic problem affecting large numbers of people internationally. While this has been a problem for decades, the Panorama programme shows that there are still almost no NHS services to support patients who have been harmed by taking medicines as prescribed by their doctor, such as antidepressants and benzodiazepines. In the absence of these services, the programme detailed how patients experiencing severe and prolonged withdrawal symptoms have resorted to online peer groups for validation, support, and safe drug tapering advice. The patient community, it seems, has been forced to develop its own withdrawal protocols (e.g. for antidepressants) in the absence of sufficient tapering guidance being advanced by NICE (The National Institute for Health and Care Excellence) or any other national clinical guidelines internationally.
. . . We believe that the NHS has a clinical and moral obligation to help those who have been harmed by taking their medication as prescribed. Panorama has revealed the scale of the problem and the horrendous impact it has had on so many peoples lives. To help mitigate these impacts, we therefore urgently call upon the UK government to fund and implement withdrawal support services, including a national helpline.
***
Back to Around the Web
The post The Government Has a Moral Duty to Help Those Harmed by Prescription Drugs appeared first on Mad In America.
04:10
The Invisible Cage Called Freedom: My Work, My Kids, My Mental Health Mad In America
I
see my 3-year-olds innocent smile as she plays and rides her tricycle, and I cant help but feel sad and worried about my daughters future. I dont want them to hurt and be disappointed in a world that can nbe so cruel and heartless.
I observe little Pheanix, whos over-the-moon as she does her exciting little dancebecause, after so many frustrating trial-and-error attempts, she finally can peddle forward smoothly and continuously. She searches for me and yells, I did it, Mama! Her voice strikes my soul, as instantaneously and simultaneously I felt warmhearted, proud, and also nostalgic because I know time will go by so quickly.
When did she get so big, so smart, so kind? What did I do to deserve her? I dont want her world to shatter, and her dreams to evaporate. I know it is my responsibility as a mother to prepare her for the disappointment of what life can be. She and her baby sister Zaphyre deserve better than what I haveand had.
I must admit something: The reason I had Pheanix was to hel...
03:29
Race and Abuse in Inpatient Settings: What Happens Behind Locked Doors Mad In America
Irvo Noel Otieno was a 28-year-old Black man who died of asphyxia due to being smothered by seven deputies in a Virginia mental hospital in March of 2023. This didnt get as much publicity as the George Floyd case and Im not even sure if it inspired any protests.
A very similar thing happened to me on multiple occasions. As a petite Mexican-American woman Ive been physically assaulted by nurses and security guards so many times that I lost count. One incident in particular that I remember happened at Oregon Health and Science University in Portland, OR, I believe in 2011 or 2012, when I refused my medication because the doctor changed the dosage without consulting me. The male nurse called in a big burly male security guard to pin me down so that the nurse could forcibly inject me with medication. I remember the doctor actually being apologetic the next day saying they should have told me about changing my medication. When I asked another security guard about it, he angrily told me that they were there to protect staff not patients.
I had forgotten this incident and thought I had left it behind in the past. But an offhand remark from my psychiatrist su...
02:58
How Do Dental Implants Work? A Guide About Dental Implants Waking Science
In spite of remarkable advances in dental care, many people still suffer from a myriad of dental conditions. These problems range from decay, periodontal diseases, and injuries, that can cost them their smiles.
The good news is dental implants can restore your dental health and get you smiling confidently once again. Dental implants look just like your natural teeth, and sometimes they look even better! You can usually get dental implants with your regular dentist.
Dental implants are made from titanium. They look like hollow screws. The second part of the implant connects the screw to the crown. This part is known as the abutment, which is also made from titanium material. The third and most visible part of the implant is the tooth itself. Although dental implants are artificial, they require as much care and attention as you give your natural teeth.
But how do dental implants work exactly? Here is a guide about the dental implants procedure:
1. Titanium Post Is Inserted Into the Jawbone
Dental implants rely on a vital component like titanium
post. It would be impossible to have a dental implant without this
component. This is because the post secures the implant in
place.
However, before affixing the titanium post, your dentist should first assess the jawbone to determine its density and quality. The jawbone should be healthy and strong enough to support and fuse the post. This forms a strong bond that cannot be broken, securing the implants.
2. Using Titanium Post, Which Is Bio-Compatible
This is the primary reason why a titanium post is
preferred when it comes to securing dental implants. The body
doesnt see titanium as a foreign object, so it doesnt reject it.
Among surgically implanted devices, dental implants are the most
compatible with the human body. They have the highest chances of
success among surgically implanted devices.
3. Implanting the Titanium Post
...
00:04
Tucker Carlson June 22 2023 on the Hatred of Candidate Kennedy Age of Autism The Rebel Alliance!
Anne Dachel has transcribed Tucker Carlson's Tucker on Twitter June 22, 2023
comments on Candidate Kennedy and Dr. Peter Hotez for posterity.
Posterity in this case is not "future generations," but anyone who
tries to find the YouTube video, which will surely be taken down
soon. Isn't Anne Dachel the GREATEST?
June 22, 2023, Tucker Carlson 6/22/23 Ep. 6
Theres never been a candidate for President the media hated more Robert F. Kennedy, Jr.
You thought that title belonged to Donald Trump. Of course, it must. But go check the coverage.
Trump got a gentle scalp massage by comparison when he announced.
When Trump rolled out his presidential campaign in 2015, the New York Times waited until the 17th paragraph of the story to attack him. But as well known as he is, the paper said at the time, Trump is also widely disliked. Then they cited a pool to back it up. That was the attack on Trump.
Eight years later, the Times attacked Bobby Kennedy in the very first sentence of the story. Robert F. Kennedy, Jr. announced a presidential campaign on Wednesday built on re-litigating COVID-19 shutdowns and shaking Americans faith in science.
Shaking Americans faith in science. Imagine if youre an ordinary New York Times subscriber reading that over your pre-war rent controlled duplex on Columbus Avenue.
Youd think Bobby Kennedy just declared war on the Enlightenment. My fellow Americans, I have come to shake your faith in science. Join me as I drag our nation back to the medieval period. Youd be appalled.
CBS News viewers likely were appalled. In its coverage of Kennedys announcement, CBS denounced the candidates views as misleading and dangerous.
The LA Times called him a thr...
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Friday, 23 June
23:38
4 Best Low Oxalate Alternatives to Almond Flour The Healthy Home Economist
Low oxalate alternatives to almond meal and other types of nut flour for keto or grain-free baking and cooking needs. My grain-free blueberry pecan cobbler food feature in the weekly newsletter elicited a number of questions for a low oxalate alternative to the nut flour portion of the recipe. Why Do Some People Avoid High
The post 4 Best Low Oxalate Alternatives to Almond Flour appeared first on The Healthy Home Economist.
21:18
Drama, Conflict and Challenges Age of Autism The Rebel Alliance!
The Real RFK Jr
biography written by Dick Russell from Skyhorse Publishing debuted
this week on June 20th. This book would be important even if
Robert Kennedy Jr was not running for the Democratic nomination for
the office of President of the United States. However, it's a
non-mainstream media way to learn more about Kennedy.
Click and buy your copies today.
Share with friends, family, Little Free Libraries. We do not
endorse candidates. We will educate, especially where their
campaign overlaps with our topics and mission.
Kennedy has been a stalwart champion for the underdog for
decades. And Lord knows, we're included in the underdog category.
The description sounds a lot like our lives, "An epic biography
filled with drama, conflict, and surmounted challenges."
From the publisher:
The Real RFK Jr. is
an intimate biographical portrait examining the controversial
activist's journey from anguish and addiction to becoming the
country's leading environmental champion fighting government
corruption, corporate greed, and a captured media. Written by his
longtime colleague Dick Russell, the biography also exposes the
misconceptions and explains the rationale behind Kennedy's campaign
to protect public health.
Provided exclusive source material, including access to
Kennedys unpublished writings and personal journals, the author
conducted dozens of interviews with him as well as numerous friends
and associates. Russell delves into everything from Kennedys
sometimes death-defying river rafting adventures to his pioneering
legal cases against polluters such as Smithfield Foods and
Monsanto, while founding the worlds largest water protection group.
The Real RFK Jr. also examines Kennedys pursuit of the truth about
the assassinations of his father and uncle, the wrongful murder
conviction of his cousin, and the false narratives around the
COVID-19 pandemic.
...
21:00
Science-Based Satire: Could Spontaneous Human Combustion Become a Chronic, Treatable Disease? Maybe. Yeah, I think so. Science-Based Medicine
Do humans spontaneously burst into flames? Can these mysterious cases be predicted and kept at bay by homeopathic remedies? No. They don't and they can't. I made it all up. Is it just me, or is getting really hot in here.
The post Science-Based Satire: Could Spontaneous Human Combustion Become a Chronic, Treatable Disease? Maybe. Yeah, I think so. first appeared on Science-Based Medicine.20:00
Plagiarism scandal engulfs high-profile academic in Latvia Retraction Watch
Two years after it was quietly retracted due to plagiarism, a paper by a prominent researcher in Latvia has set the countrys media ablaze, drawing comments from, among others, the minister of education and science and the rector of a leading university.
The plagiarized paper came to public attention in March when the Latvian magazine Ir published serious allegations against Maris Klavins, a professor and former dean at the University of Latvia. The allegations concern possible fraud in an EU-funded project headed by Klavins and include not only plagiarism but data falsification, budget irregularities and suspicious cash payments.
According to Ir, the Latvian police have opened a criminal investigation into the case. Klavins told the magazine he has not been charged in the matter.
In an email to Retraction Watch, Klavins called the allegations lies and said the person responsible for my defamation and persecution was his former PhD student Dmitrijs Porsnovs.
Presently this case is under investigation within a legal process, Klavins added. Considering this, I cannot give comments on the case.
According to the article in Ir, Porsnovs discovered in 2021 that parts of an internal report he had written about the processing of algae for energy production had been published earlier that year by Klavins and others in the journal Agronomy Research without listing him as an author or citing his work. Porsnovs, who is now a graduate student at the University of Stavanger in Norway, did not respond to requests for comments.
The magazine article includes examples of similar text and identical data in Porsnovs report and Klavins paper, titled Algae processing for energy production: development of waste pyrolysis technology. Ir also...
13:12
Rosemary extract protects liver mitochondria from oxidative damage and prevents acetaminophen-induced hepatotoxicity. GreenMedInfo
PMID: Antioxidants (Basel). 2023 Mar 3 ;12(3). Epub 2023 Mar 3. PMID: 36978874 Abstract Title: Rosemary (L.) Glycolic Extract Protects Liver Mitochondria from Oxidative Damage and Prevents Acetaminophen-Induced Hepatotoxicity. Abstract: L. (rosemary) is an aromatic culinary herb. Native to the Mediterranean region, it is currently cultivated worldwide. In addition to its use as a condiment in food preparation and in teas, rosemary has been widely employed in folk medicine and cosmetics. Several beneficial effects have been described for rosemary, including antimicrobial and antioxidant activities. Here, we investigated the mechanisms accounting for the antioxidant activity of the glycolic extract of() in isolated rat liver mitochondria (RLM) under oxidative stress conditions. We also investigated its protective effect against acetaminophen-induced hepatotoxicity in vivo. A crude extract was obtained by fractionated percolation, using propylene glycol as a solvent due to its polarity and cosmeceutical compatibility. The quantification of substances with recognized antioxidant action revealed the presence of phenols and flavonoids. Dereplication studies carried out through LC-MS/MS and GC-MS, supported by The Global Natural Product Social Molecular Networking (GNPS) platform, annotated several phenolic compounds, confirming the previous observation. In accordance,decreased the production of reactive oxygen species (ROS) elicited by Feor-BOOH and inhibited the lipid peroxidation of mitochondrial membranes in a concentration-dependent manner in RLM. Such an effect was also observed in liposomes as membrane models.also prevented the oxidation of mitochondrial protein thiol groups and reduced glutathione (GSH). In model systems,exhibited a potent scavenger activity toward 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals and superoxide anions. It also demonstrated an Fechelating activity. Moreover,did not exhibit cytotoxicity or dissipate the mitochondrial membrane potential () in rat liver fibroblasts (BRL3A cells). To evaluate whether such antioxidant protective activity observed in vitro could also be achieved in vivo, a well-established model of hepatotoxicity induced by acute exposure to acetaminophen (AAP) was used. This model depletes GSH and promotes oxidative-stress-mediated tissue damage. The treatment of rats with 0.05%, administered intraperitoneally for four days, resulted in inhibition of AAP-induced lipid peroxidation of the liver and the prevention of hepatotoxicity, maintaining alanine and aspartate aminotransferase (ALT/AST) levels equal to those of the normal, non-treated rats. Together, these findings highlight the potent antioxidant activity of rosemary, which is able to protect mitochondria from oxidative damage in vitro, and effects such as the antioxidant and h...
13:04
Carnosic acid protects against doxorubicin-induced cardiotoxicity. GreenMedInfo
PMID: Food Funct. 2023 Apr 24 ;14(8):3849-3862. Epub 2023 Apr 24. PMID: 37013966 Abstract Title: Carnosic acid protects against doxorubicin-induced cardiotoxicity through enhancing the Nrf2/HO-1 pathway. Abstract: Doxorubicin (DOX) is used extensively in anticancer therapy, but its clinical application is limited due to its cardiotoxicity. Carnosic acid (CA) is a bioactive compound found in rosemary. It has been shown to reduce inflammation and reactive oxygen species. The purpose of this study was to investigate the potential cardioprotective effects of CA in response to DOX-induced cardiotoxicity. Here, C57BL/6 mice were administered an intraperitoneal injection of DOX (5 mg kg, ip) once a week for three consecutive weeks and treated with CA (40 mg kg, ig) for a three-week experimental period. Forstudy, neonatal rat ventricular cardiomyocytes were used to validate the protective effects of CA (20M) in response to DOX-induced cardiotoxicity. CA markedly suppressed oxidative stress, apoptosis, and pyroptosis responses in the mouse hearts, eventually improving cardiac function. CA showed its antioxidant effect by activating nuclear factor erythroid 2-related factor (Nrf2) and its downstream heme oxygenase-1 (HO-1); CA also reduced oxidative stress by lowering the MDA and lipid ROS levels and raising the SOD and GSH-px levels. Additionally, CA treatment significantly increased Bcl-2 and inhibited Bax and Caspase-3 cleavage in DOX-induced cardiotoxicity. Moreover, CA suppressed the NOD-like receptor protein 3 (NLRP3) pathway to mitigate pyroptosis, as evidenced by lowered caspase1, interleukin-18, and interleukin-1. Consistently, the transfection of Nrf2-siRNA eliminated the protective effects of CA on cardiomyocytes. Altogether, our findings demonstrated that CA inhibited NLRP3 inflammasomesactivating the Nrf2-related cytoprotective system and protected the heart from oxidative damage, apoptosis, and pyroptosis, implying that the use of CA could be a potential therapeutic strategy in the prevention of DOX-associated myocardiopathy.
12:33
Carnosic acid ameliorates indomethacin-induced gastric ulceration. GreenMedInfo
PMID: Biomedicines. 2023 Mar 9 ;11(3). Epub 2023 Mar 9. PMID: 36979808 Abstract Title: Carnosic Acid Ameliorates Indomethacin-Induced Gastric Ulceration in Rats by Alleviating Oxidative Stress and Inflammation. Abstract: Nonsteroidal anti-inflammatory drugs (NSAIDs) such as aspirin and indomethacin (IND) are the most commonly prescribed for inflammation or pain. However, widespread use causes several adverse effects, such as gastric ulcers, upper gastric system bleeding, and erosions. Carnosic acid (CA) is an important natural antioxidant found in rosemary (Rosmarinus essentials) and exhibits a protective effect by suppressing oxidative stress and inflammation. This study aimed to investigate the impact of CA on IND-induced gastric ulceration. Wistar male rats received CA (100 mg/kg) or esomeprazole (ESP) (20 mg/kg, standard drug) by oral gavage for 14 days, after that gastric ulceration was induced by oral administration of 100 mg/kg IND. CA pretreatment attenuated both gross morphological lesions and histopathological alterations. CA strongly reduced IND-induced oxidative stress, verified by a decrease in MDA (
12:13
Anti- heliobacter pylori and anti-Inflammatory potential of Salvia officinalis metabolites. GreenMedInfo
PMID: Metabolites. 2023 Jan 16 ;13(1). Epub 2023 Jan 16. PMID: 36677061 Abstract Title: Anti-and Anti-Inflammatory Potential ofMetabolites: In Vitro and In Silico Studies. Abstract: Due to its rising antibiotic resistance and associated inflammations,poses a challenge in modern medicine., a member of the Lamiaceae family, is a promising medicinal herb. In this regard, a phytochemical screening followed by GC-MS and LC-MS was done to evaluate the chemical profile of the total ethanolic extract (TES) and the essential oil, respectively. The anti-and the anti-inflammatory activities were evaluated by a micro-well dilution technique and COX-2 inhibition assay. Potential anti-inhibitors were determined by an in silico study. The results revealed that the main metabolites were flavonoids, sterols, volatile oil, saponins, and carbohydrates. The LC-MS negative ionization mode demonstrated 12 compounds, while GC-MS showed 21 compounds. Carnosic acid (37.66%), epirosmanol (20.65%), carnosol1 (3.3%), and 12--methyl carnosol (6.15%) were predominated, while eucalyptol (50.04%) and camphor (17.75%) were dominant in LC-MS and GC-MS, respectively. TES exhibited the strongest anti-activity (3.9g/mL) asymptotic to clarithromycin (0.43g/mL), followed by the oil (15.63g/mL). Carnosic acid has the best-fitting energy to inhibit(-46.6769 Kcal/mol). TES showed the highest reduction in Cox-2 expression approaching celecoxib with IC= 1.70.27g/mL, followed by the oil with IC= 5.30.62g/mL. Our findings suggest thatmetabolites with anti-inflammatory capabilities could be useful inmanagement. Further in vivo studies are required to evaluate and assess its promising activity.
12:05
ALS-L1023 from Melissa officinalis alleviates liver fibrosis in a non-alcoholic fatty liver disease model. GreenMedInfo
PMID: Life (Basel). 2022 Dec 29 ;13(1). Epub 2022 Dec 29. PMID: 36676050 Abstract Title: ALS-L1023 fromAlleviates Liver Fibrosis in a Non-Alcoholic Fatty Liver Disease Model. Abstract: ALS-L1023 is an ingredient extracted fromL. (Labiatae; lemon balm), which is known as a natural medicine that suppresses angiogenesis. Herein, we aimed to determine whether ALS-L1023 could alleviate liver fibrosis in the non-alcoholic fatty liver disease (NAFLD) model. C57BL/6 wild-type male mice (age, 6 weeks old) were fed a choline-deficient high-fat diet (CDHFD) for 10 weeks to induce NAFLD. For the next 10 weeks, two groups of mice received the test drug along with CDHFD. Two doses (a low dose, 800 mg/kg/day; and a high dose, 1200 mg/kg/day) of ALS-L1023 were selected and mixed with feed for administration. Obeticholic acid (OCA; 10 mg/kg/day) was used as the positive control. Biochemical analysis revealed that the ALS-L1023 low-dose group had significantly decreased alanine transaminase and aspartate transaminase. The area of fibrosis significantly decreased due to the administration of ALS-L1023, and the anti-fibrotic effect of ALS-L1023 was greater than that of OCA. RNA sequencing revealed that the responder group had lower expression of genes related to the hedgehog-signaling pathway than the non-responder group. ALS-L1023 may exert anti-fibrotic effects in the NAFLD model, suggesting that it may provide potential benefits for the treatment of liver fibrosis.
12:02
Antiviral potential of Melissa officinalis L.: A literature review. GreenMedInfo
PMID: Nutr Metab Insights. 2023 ;16:11786388221146683. Epub 2023 Jan 12. PMID: 36655201 Abstract Title: Antiviral Potential of Melissa officinalis L.: A Literature Review. Abstract: The use of synthetic drugs has increased in recent years; however, herbal medicine is yet more trusted among a huge population worldwide; This could be due to minimal side effects, affordable prices, and traditional beliefs. Lemongrass () has been widely used for reducing stress and anxiety, increasing appetite and sleep, reducing pain, healing wounds, and treating poisonous insect bites and bee stings for a long time. Today, research has shown that this plant can also fight viruses including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Herpes Simplex Virus (HSV), and Human Immunodeficiency Virus (HIV) through various mechanisms such as inhibiting HSV-1 from binding to host cell, inhibiting HSV-1 replication during the post-adsorption or inhibiting main protease and spike protein of SARS-CoV-2, furthermore, be effective in treating related diseases. This Review investigated the antiviral properties ofand its effect on viral diseases. More in vitro and in vivo studies are needed to determineunderlying mechanism, and more randomized controlled trials should be done to identify its effect in humans. Also, due to the usefulness and lack of side effects, it can be used more as a complementary medicine.
11:59
Melissa Officinalis L. aqueous extract pretreatment decreases methotrexate-induced hepatotoxicity. GreenMedInfo
PMID: Ann Nucl Med. 2023 Mar ;37(3):166-175. Epub 2022 Dec 5. PMID: 36469234 Abstract Title: Melissa Officinalis L. aqueous extract pretreatment decreases methotrexate-induced hepatotoxicity at lower dose and increasesTc-phytate liver uptake, as a probe of liver toxicity assessment, in rats. Abstract: OBJECTIVE: Hepatotoxicity remains amongst the restricting factors of Methotrexate (MTX)-associated cancer therapy, especially in high doses of chemo-drugs or prolonged treatment. Due to the known protective effects of Melissa officinalis (M. officinalis), the aqueous extract of this plant was evaluated to ameliorate MTX-associated hepatotoxicity in rats.METHODS: Adult female Wistar rats were received or not M. officinalis aqueous extract at doses of 100 mg/kg (for 14 and 24 consecutive days) and 2 g/kg (for 14 consecutive days) by gavage technique. MTX (20 mg/kg) was intraperitoneally injected on the 10th- and 20th-day post-M. officinalis treatment. 24 h after the last day of treatment,Tc-phytate was intravenously injected through the tail of rats. Animals were killed at 20 min after radiocolloid injection, and vital tissues including the liver and spleen were isolated, weighed, and their radioactivity was counted. As well,Tc-phytate scintigraphy and histopathology of the liver were performed for higher accuracy.RESULT: A significant increase in liver radioactivity was detected in M. officinalis+MTX receiving groups compared with the MTX rats which were more robust at a dose of 100 mg/kg for 14 days. Also, a significant reduction in liver radioactivity was evident with M. officinalis extract at a dose of 2 g/kg for 14 days in comparison with the control group, this reduction was not significant at the lower dose of 100 mg/kg. Gamma scintigraphy and histopathological examinations confirmed the hepatoprotective effect of M. officinalis vs MTX-induced liver injury in rats.CONCLUSION: In conclusion, we highlighted the liver uptake ofTc-phytate as a valuable method for assessment of liver toxicity and addressed that M. officinalis pretreatment (100 mg/kg for 14 days) ameliorates the MTX-associated hepatotoxicity in rats; however, M. officinalis itself induces liver toxicity at higher doses.
11:57
Cytotoxic effect of the crude alcoholic extract of the fruits of Citrullus colocynthis on human hepatocyte carcinoma. GreenMedInfo
PMID: Arch Razi Inst. 2022 Aug ;77(4):1389-1395. Epub 2022 Aug 31. PMID: 36883161 Abstract Title: Cytotoxic Effect of the Crude Alcoholic Extract of the Fruits ofon Human Hepatocyte Carcinoma (Hep-G2). Abstract: Theis a perennial herbaceous plant belonging to the family. Several pharmacological investigations have been performed based on the medicinal application of. The anticancer and antidiabetic activities of fruit and seed extracts ofhave been studied. Newly developed anticancer/antitumor medications appear to have been developed based on the extracted chemicals fromdue to the high contents of cucurbitacins. The present study aimed to identify the cytotoxic effect of the crude alcoholic extract of plants ofon the growth of human hepatocyte carcinoma (Hep-G2). The results of the chemical (preliminary) examination of the extract indicated that the fruits contain most of the secondary metabolites including Flavonoids, Tannins, Sapiens, Resins, Amino acids, Glycosides, Terpenes, Alkaloids, and Flavonoids. The toxicological effect of the crude extract was investigated by using six half dilutions concentrations of 20,10,5,2.5,1.25, and 0.625g/m at three exposure periods of 24,48, and 72 h using MTT testing. The toxicological effect of the extract appeared for all six concentrations in the Hep-G2 cell line. The highest concentration of 20g/ml had the highest percentage inhibition rate with a significant difference (0.01) and reached 93.361.61 after 72 h of exposure. While the lowest concentration of 0.625g/ml was recorded rate of inhibition of 23.362.34 after 24 h of exposure. The findings of the present study concluded that theis one of the most promising medicinal plants which effectively treats cancer through its inhibitory effect and fatal toxicity on cancer cells.
11:53
A natural glucan from black bean inhibits cancer cell proliferation. GreenMedInfo
PMID: Molecules. 2023 Feb 19 ;28(4). Epub 2023 Feb 19. PMID: 36838963 Abstract Title: A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway. Abstract: A natural-1,6-glucan named BBWPW was identified from black beans. Cell viability assay showed that BBWPW inhibited the proliferation of different cancer cells, especially HeLa cells. Flow cytometry analysis indicated that BBWPW suppressed the HeLa cell cycle in the G2/M phase. Consistently, RT-PCR experiments displayed that BBWPW significantly impacts the expression of four marker genes related to the G2/M phase, including,,, and. To explore the molecular mechanism of BBWPW to induce cell cycle arrest, a transcriptome-based target inference approach was utilized to predict the potential upstream pathways of BBWPW and it was found that the PI3K-Akt and MAPK signal pathways had the potential to mediate the effects of BBWPW on the cell cycle. Further experimental tests confirmed that BBWPW increased the expression ofandand decreased the expression ofandThese results suggested that BBWPW could regulate the PI3K-Akt and MAPK pathways to induce cell cycle arrest and ultimately inhibit the proliferation of HeLa cells, providing the potential of the black bean glucan to be a natural anticancer drug.
11:49
Apigenin alleviates endoplasmic reticulum stress-mediated apoptosis in INS-1 -cells. GreenMedInfo
PMID: Biol Pharm Bull. 2023 ;46(4):630-635. PMID: 37005308 Abstract Title: Apigenin Alleviates Endoplasmic Reticulum Stress-Mediated Apoptosis in INS-1-Cells. Abstract: The improvement of type 2 diabetes mellitus induced by naturally occurring polyphenols, known as flavonoids, has received considerable attention. However, there is a dearth of information regarding the effect of the trihydroxyflavone apigenin on pancreatic-cell function. In the present study, the anti-diabetic effect of apigenin on pancreatic-cell insulin secretion, apoptosis, and the mechanism underlying its anti-diabetic effects, were investigated in the INS-ID-cell line. The results showed that apigenin concentration-dependently facilitated 11.1-mM glucose-induced insulin secretion, which peaked at 30M. Apigenin also concentration-dependently inhibited the expression of endoplasmic reticulum (ER) stress signaling proteins, CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) and cleaved caspase-3, which was elevated by thapsigargin in INS-1D cells, with peak suppression at 30M. This was strongly correlated with the results of flow cytometric analysis of annexin V/propidium iodide (PI) staining and DNA fragmentation analysis. Moreover, the increased expression of thioredoxin-interacting protein (TXNIP) induced by thapsigargin was remarkably reduced by apigenin in a concentration-dependent manner. These results suggest that apigenin is an attractive candidate with remarkable and potent anti-diabetic effects on-cells, which are mediated by facilitating glucose-stimulated insulin secretion and preventing ER stress-mediated-cell apoptosis, the latter of which may be possibly mediated by reduced expression of CHOP and TXNIP, thereby promoting-cell survival and function.
11:39
The obtained results highlight that flavonoids may be a useful tool in overcoming antibiotic resistance. GreenMedInfo
PMID: Ann Agric Environ Med. 2023 Mar 31 ;30(1):61-64. Epub 2023 Feb 27. PMID: 36999857 Abstract Title: Antimicrobial synergistic effects of apigenin, (-)-epigallocatechin-3-gallate, myricetin and luteolin in combination with some antibiotics. Abstract: INTRODUCTION AND OBJECTIVE: Antimicrobial resistance, which is considered one of the most important problems of the 21 st century, brings many problems with it, such as increasing mortality rates and treatment costs. Difficulties in the treatment of infections caused by resistant microorganisms have led to the search and need for new antimicrobials or new molecules that interact synergistically with antimicrobials. The aim of this study is to investigate whether various flavonoids have synergistic effects with some antibiotics.MATERIAL AND METHODS: During this study, standard bacterial strainsATCC 25922,ATCC 700603,ATCC 9027,ATCC 29213 andATCC 43300 were used. Minimal inhibitory concentrations of all antibiotics and flavonoids were found by the broth microdilution method. Interactions between antibiotics and flavonoids were then determined by using the checkerboard method. Interactions between antibiotics and flavonoids were evaluated according to the FIC index (FIC) results.RESULTS: According to the results of the microdilution test, the bacterial strains used in this study (except for MRSA) were generally sensitive to antibiotics. Interaction study results showed promising results regarding the synergistic interactions of antibiotics with flavonoids. Epigallocatechin gallate and luteolin especially showed synergistic interaction with antibiotics in many microorganisms. It was found that myricetin showed synergistic interaction only with levofloxacin. Likewise, it was detected that apigenin had limited synergistic interaction with antibiotics.CONCLUSIONS: The obtained results highlight that flavonoids may be a useful tool in overcoming antibiotic resistance.
11:19
Kaempferol ameliorates pulmonary vascular remodeling in chronic hypoxia-induced pulmonary hypertension. GreenMedInfo
PMID: Toxicol Appl Pharmacol. 2023 May 1 ;466:116478. Epub 2023 Mar 20. PMID: 36940862 Abstract Title: Kaempferol ameliorates pulmonary vascular remodeling in chronic hypoxia-induced pulmonary hypertension rats via regulating Akt-GSK3-cyclin axis. Abstract: Excessive proliferation of pulmonary artery smooth muscle cells (PASMCs) is considered a major contributor to elevated pulmonary vascular resistance and a key mechanism of vascular remodeling in hypoxia-induced pulmonary hypertension (HPH). Kaempferol is a natural flavonoid compound and can be derived from numerous common medicinal herbs and vegetables, which exhibit antiproliferative and proapoptotic properties, however, the effects of kaempferol on vascular remodeling in HPH remain unexplored. In this study, SD rats were placed in a hypobaric hypoxia chamber for four weeks to establish a pulmonary hypertension model and given either kaempferol or sildenafil (an inhibitor of PDE-5) during days 1-28, after which the hemodynamic parameter and pulmonary vascular morphometry were assessed. Furthermore, primary rat PASMCs were exposed to hypoxic conditions to generate a cell proliferation model, then incubated with either kaempferol or LY294002 (an inhibitor of PI3K). Immunoblotting and real-time quantitative PCR assessed the protein and mRNA expression levels in HPH rat lungs and PASMCs. We found that kaempferol reduced pulmonary artery pressure and pulmonary vascular remodeling, and alleviated right ventricular hypertrophy in HPH rats. The mechanistic analysis demonstrated that kaempferol reduced the protein levels of phosphorylation of Akt and GSK3, leading to decreased expression of pro-proliferation (CDK2, CDK4, Cyclin D1, and PCNA) and anti-apoptotic related proteins (Bcl-2) and increased expression of pro-apoptosis proteins (Bax and cleaved caspase 3). These results collectively demonstrate that kaempferol ameliorates HPH in rats by inhibiting PASMC proliferation and pro-apoptosis via modulation of the Akt/GSK3/CyclinD axis.
11:15
Quercetin modulates signal transductions and targets non-coding RNAs against cancer development. GreenMedInfo
PMID: Cell Signal. 2023 Jul ;107:110667. Epub 2023 Apr 5. PMID: 37023996 Abstract Title: Quercetin modulates signal transductions and targets non-coding RNAs against cancer development. Abstract: In recent decades, various investigations have indicated that natural compounds have great potential in the prevention and treatment of different chronic disorders including different types of cancer. As a bioactive flavonoid, Quercetin (Qu) is a dietary ingredient enjoying high pharmacological values and health-promoting effects due to its antioxidant and anti-inflammatory characterization. Conclusive in vitro and in vivo evidence has revealed that Qu has great potential in cancer prevention and development. Qu exerts its anticancer influences by altering various cellular processes such as apoptosis, autophagy, angiogenesis, metastasis, cell cycle, and proliferation. In this way, Qu by targeting numerous signaling pathways as well as non-coding RNAs regulates several cellular mechanisms to suppress cancer occurrence and promotion. This review aimed to summarize the impact of Qu on the molecular pathways and non-coding RNAs in modulating various cancer-associated cellular mechanisms.
11:13
Quercetin downregulates the expression of IL15 in cancer cells through DNA methylation. GreenMedInfo
PMID: Eur Rev Med Pharmacol Sci. 2023 Mar ;27(6):2580-2590. PMID: 37013776 Abstract Title: Quercetin downregulates the expression of IL15 in cancer cells through DNA methylation. Abstract: OBJECTIVE: This study aimed to investigate the effect of quercetin on cellular immunity (via IL15 expression) against cancer and to elucidate its regulatory mechanism.MATERIALS AND METHODS: HeLa cells and A549 cells were cultured in vitro and were divided into control (DMSO treated) and experimental groups (treated with different concentrations of quercetin). Transcript levels of IL15 and DNA methyltransferase (DNMTS) were measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Genomic DNA was extracted, treated with bisulfite, and the promoter region of IL15 was cloned. Finally, Sanger sequencing was used to detect the degree of promoter methylation.RESULTS: Following quercetin treatment, the expression of IL15 was significantly downregulated in HeLa and A549 cells. The methylation level of IL15 promoter in HeLa cells was about twice that of the control group, and the methylation level of IL15 promoter in A549 cells was about three times that of the control group.CONCLUSIONS: Quercetin inhibits cancer cell proliferation while downregulating IL15 expression, and this regulation is achieved by increasing the methylation of the IL15 promoter.
11:11
Protective effects of querectin against MPP+-induced dopaminergic neurons injury. GreenMedInfo
PMID: Front Biosci (Landmark Ed). 2023 Mar 2 ;28(3):42. PMID: 37005755 Abstract Title: Protective Effects of Querectin against MPP-Induced Dopaminergic Neurons Injury via the Nrf2 Signaling Pathway. Abstract: BACKGROUND: Parkinson's disease (PD) is a common selective and progressive neurodegenerative disorder of nigrostriatal dopaminergic (DA) neurons. Quercetin is a bioflavonoid with antioxidant, anti-inflammatory, anti-aging and anti-cancer properties. However, the exact mechanism by which quercetin exerts its protective effect on DAergic neurons remains unclear.PURPOSE: To investigate the underlying molecular mechanism of quercetin's protective effect on DA neurons using 1-methyl-4-phenylpyridinium (MPP+)-induced PD ferroptosis model.METHODS: MPP+ was used to induce cytotoxicity in SH-SY5Y/primary neurons. Cell viability and apoptosis were assessed by CCK-8 assay and flow cytometry. The expression levels of ferroptosis-related proteins (NCOA4, SLC7A11, Nrf2, and GPX4) were determined by Western blotting. Malondialdehyde (MDA), iron, and GPX4 levels were assesed using corresponding assay kits. Lipid peroxidation was assessed by C11-BODIPY staining.RESULTS: In the MPP+-induced ferroptosis model of SH-SY5Y cells, the expressions of SLC7A11 and GPX4 were inhibited, and the expression of NCOA4 protein was increased, causing the overproduction of MDA and lipid peroxidation. Quercetin can reduce the above changes caused by MPP+, that is, reduce the protein expression of NCOA4 in SH-SY5Y cells, increase SLC7A11 and GPX4 partially inhibited by MPP+, and reduce MDA overproduction and lipid peroxidation to protect DA neurons. Nrf2 inhibitor ML385 could inhibit quercetin-induced increase of GPX4 and SLC7A11 protein expression, indicating that the protective effect of quercetin was mediated through Nrf2.CONCLUSIONS: The results of this study suggest that quercetin regulates ferroptosis through Nrf2-dependent signaling pathways, thereby inhibiting MPP+-induced neurotoxicity in SH-SY5Y/primary neurons.
11:07
In vitro and molecular docking analysis of quercetin as an anti-inflammatory and antioxidant. GreenMedInfo
PMID: Curr Pharm Des. 2023 ;29(11):883-891. PMID: 37005541 Abstract Title: and Molecular Docking Analysis of Quercetin as an Anti-inflammatory and Antioxidant. Abstract: INTRODUCTION: Quercetin (3,3',4',5,7-pentahydroxyflavone) is a dietary flavonoid with good antioxidant and anti-inflammatory properties.AIMS: The present study aims to determine these effects in peripheral blood mononuclear cells (PBMCs) evoked by lipopolysaccharides (LPS).METHODS: The mRNA expression and protein secretion of inflammatory mediators were evaluated by enzyme- linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (PCR), respectively. Western blotting was utilized for assessing p65-NF-B phosphorylation. Ransod kits evaluated the glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity in the cell lysates. Ultimately, the molecular docking approach was performed to investigate the biological activity of Quercetin against NF-B pathway proteins and antioxidant enzymes.RESULTS: The findings revealed that quercetin significantly attenuated the expression and secretion of inflammatory mediators and p65-NF-B phosphorylation in LPS-induced PBMCs. Additionally, quercetin dose-dependently improved the activities of SOD and GPx enzymes and decreased LPS-mediated oxidative stress in PBMCs. Moreover, quercetin has a considerable binding affinity to IKb, the core element of the NF-B pathway and the antioxidant enzyme SOD.CONCLUSION: The data show that quercetin plays a vital role in ameliorating inflammation and oxidative stress caused by LPS in PBMCs.
11:02
Quercetin and its nano-formulations for brain tumor therapy. GreenMedInfo
PMID: Pharmaceutics. 2023 Mar 16 ;15(3). Epub 2023 Mar 16. PMID: 36986827 Abstract Title: Quercetin and Its Nano-Formulations for Brain Tumor Therapy-Current Developments and Future Perspectives for Paediatric Studies. Abstract: The development of efficient treatments for tumors affecting the central nervous system (CNS) remains an open challenge. Particularly, gliomas are the most malignant and lethal form of brain tumors in adults, causing death in patients just over 6 months after diagnosis without treatment. The current treatment protocol consists of surgery, followed using synthetic drugs and radiation. However, the efficacy of these protocols is associated with side effects, poor prognosis and with a median survival of fewer than two years. Recently, many studies were focused on applying plant-derived products to manage various diseases, including brain cancers. Quercetin is a bioactive compound derived from various fruits and vegetables (asparagus, apples, berries, cherries, onions and red leaf lettuce). Numerous in vivo and in vitro studies highlighted that quercetin through multitargeted molecular mechanisms (apoptosis, necrosis, anti-proliferative activity and suppression of tumor invasion and migration) effectively reduces the progression of tumor cells. This review aims to summarize current developments and recent advances of quercetin's anticancer potential in brain tumors. Since all reported studies demonstrating the anti-cancer potential of quercetin were conducted using adult models, it is suggested to expand further research in the field of paediatrics. This could offer new perspectives on brain cancer treatment for paediatric patients.
10:51
Quercetin and its fermented extract as a potential inhibitor of bisphenol A-exposed HT-29 colon cancer cells' viability. GreenMedInfo
PMID: Int J Mol Sci. 2023 Mar 15 ;24(6). Epub 2023 Mar 15. PMID: 36982678 Abstract Title: Quercetin and Its Fermented Extract as a Potential Inhibitor of Bisphenol A-Exposed HT-29 Colon Cancer Cells' Viability. Abstract: Bisphenol A (BPA) promotes colon cancer by altering the physiological functions of hormones. Quercetin (Q) can regulate signaling pathways through hormone receptors, inhibiting cancer cells. The antiproliferative effects of Q and its fermented extract (FEQ, obtained by Q gastrointestinal digestion and in vitro colonic fermentation) were analyzed in HT-29 cells exposed to BPA. Polyphenols were quantified in FEQ by HPLC and their antioxidant capacity by DPPH and ORAC. Q and 3,4-dihydroxyphenylacetic acid (DOPAC) were quantified in FEQ. Q and FEQ exhibited antioxidant capacity. Cell viability with Q+BPA and FEQ+BPA was 60% and 50%, respectively; less than 20% of dead cells were associated with the necrosis process (LDH). Treatments with Q and Q+BPA induced cell cycle arrest in the G0/G1 phase, and FEQ and FEQ+BPA in the S phase. Compared with other treatments, Q positively modulatedandgenes. Using a gene microarray of thepathway, Q, Q+BPA, FEQ and FEQ+BPA positively modulated genes involved in apoptosis and cell cycle arrest; bisphenol inhibited the expression of pro-apoptotic and cell cycle repressor genes. In silico analyses demonstrated the binding affinity of Q>BPA>DOPAC molecules for ERand ER. Further studies are needed to understand the role of disruptors in colon cancer.
10:48
Quercetin reprograms immunometabolism of macrophages ameliorated lipopolysaccharide-induced oxidative damage. GreenMedInfo
PMID: Int J Mol Sci. 2023 Mar 14 ;24(6). Epub 2023 Mar 14. PMID: 36982615 Abstract Title: Quercetin Reprograms Immunometabolism of Macrophages via the SIRT1/PGC-1Signaling Pathway to Ameliorate Lipopolysaccharide-Induced Oxidative Damage. Abstract: The redox system is closely related to changes in cellular metabolism. Regulating immune cell metabolism and preventing abnormal activation by adding antioxidants may become an effective treatment for oxidative stress and inflammation-related diseases. Quercetin is a naturally sourced flavonoid with anti-inflammatory and antioxidant activities. However, whether quercetin can inhibit LPS-induced oxidative stress in inflammatory macrophages by affecting immunometabolism has been rarely reported. Therefore, the present study combined cell biology and molecular biology methods to investigate the antioxidant effect and mechanism of quercetin in LPS-induced inflammatory macrophages at the RNA and protein levels. Firstly, quercetin was found to attenuate the effect of LPS on macrophage proliferation and reduce LPS-induced cell proliferation and pseudopodia formation by inhibiting cell differentiation, as measured by cell activity and proliferation. Subsequently, through the detection of intracellular reactive oxygen species (ROS) levels, mRNA expression of pro-inflammatory factors and antioxidant enzyme activity, it was found that quercetin can improve the antioxidant enzyme activity of inflammatory macrophages and inhibit their ROS production and overexpression of inflammatory factors. In addition, the results of mitochondrial morphology and mitochondrial function assays showed that quercetin could upregulate the mitochondrial membrane potential, ATP production and ATP synthase content decrease induced by LPS, and reverse the mitochondrial morphology damage to a certain extent. Finally, Western blotting analysis demonstrated that quercetin significantly upregulated the protein expressions of SIRT1 and PGC-1, that were inhibited by LPS. And the inhibitory effects of quercetin on LPS-induced ROS production in macrophages and the protective effects on mitochondrial morphology and membrane potential were significantly decreased by the addition of SIRT1 inhibitors. These results suggested that quercetin reprograms the mitochondria metabolism of macrophages through the SIRT1/PGC-1signaling pathway, thereby exerting its effect of alleviating LPS-induced oxidative stress damage.
10:40
C. papaya was capable of restoring the altered levels in the hepatic tissues of T2DM rats. GreenMedInfo
PMID: Toxics. 2023 Mar 1 ;11(3). Epub 2023 Mar 1. PMID: 36977005 Abstract Title: Hypoglycemic Potential ofin Liver Is Mediated through IRS-2/PI3K/SREBP-1c/GLUT2 Signaling in High-Fat-Diet-Induced Type-2 Diabetic Male Rats. Abstract: Regardless of socioeconomic or demographic background, the prevalence of type 2 diabetes mellitus, which affects more than half a billion people worldwide, has been steadily increasing over time. The health, emotional, sociological, and economic well-being of people would suffer if this number is not successfully handled. The liver is one of the key organs accountable for sustaining metabolic balance. Elevated levels of reactive oxygen species inhibit the recruitment and activation of IRS-1, IRS-2, and PI3K-Akt downstream signaling cascade. These signaling mechanisms reduce hepatic glucose absorption and glycogenesis while increasing hepatic glucose output and glycogenolysis. In our work, an analysis of the molecular mechanism ofin mitigating hepatic insulin resistance in vivo and in silico was carried out. The gluconeogenic enzymes, glycolytic enzymes, hepatic glycogen tissue concentration, oxidative stress markers, enzymatic antioxidants, protein expression of IRS-2, PI3K, SREBP-1C, and GLUT-2 were evaluated in the liver tissues of high-fat-diet streptozotocin-induced type 2 diabetic rats using q-RT-PCR as well as immunohistochemistry and histopathology. Upon treatment,restored the protein and gene expression in the liver. In the docking analysis, quercetin, kaempferol, caffeic acid, and p-coumaric acid present in the extract were found to have high binding affinities against IRS-2, PI3K, SREBP-1c, and GLUT-2, which may have contributed much to the antidiabetic property of. Thus,was capable of restoring the altered levels in the hepatic tissues of T2DM rats, reversing hepatic insulin resistance.
10:00
Will DARPA Make a Travel Adapter for Human Body? Articles
Another day, another bit of great news, folks. Everyones favorite agency, DARPA, is promising to develop a "travel adapter" for the human body. The travel adapter, intended for "warfighters," is supposed to play a magical trick on biology and program the soldiers sleep cycles at will, as well as disinfect food and water in the soldiers belly in real time. The miracle is going to be a transient and non-genetic "implantable or ingestible bioelectronic carrier."
Certainly, nothing can go wrong, and the proof is this very professionally done image on their website that shows us how one can "program" physiology by adjusting a slider in an app. This image alone is a masterpiece of seduction, selling the appealing-but-totally-fictional concept of getting "something for nothing," as if pulling it out of a magicians hat.
Betraying the Soldiers
Before we get down to the nitty-gritty of DARPAs ADAPTER program yes, the name of the program is acronym but they also call the device an "adapter," DARPA word weavers are crafty like that! I want to express my indignation at the entire vampire-like model of sacrificing human beings to senseless wars.
These wars are fought primarily to make a small crew of shameless people obscenely rich, and the biggest "beneficiaries" of the cruelty dont fight in those wars themselves, just organize the dark business of shedding other peoples blood.
Such betrayal! A lot of people on the ground join the military for noble reasons. Yes, some join out of desperation, to lift themselves out of poverty but many join for very sacred reasons, out of courage, to protect their own.
And then those brave young people get dragged into treacherous carnage, maimed in body and spirit, and sometimes experimented on (here is one pre-2020 example of the U.S. military experimenting on its soldiers, here is another, and we know what happened with COVID "vaccines").
Bioweapon Experimentation on Soldiers
Here is a phenomenally frank 2015 paper titled, "Science wars How much risk should soldiers be exposed to in military experimentation?"
The paper argues that because the warfare is shifting toward the use of bioweapons, it is appropriate to experiment on soldiers as they have already agreed to risk their lives for the benefit of the state:
"With the threat of biological war becoming a more and more dis...
The Benefits of Curcumin in Tumor Treatments Articles
Editor's Note: This article is a reprint. It was originally published March 2, 2014.
Cancer is one of the leading causes of death. What if there were a safe, natural herb that could work for nearly every type of cancer?
According to Dr. William LaValley, who focuses most of his clinical work on the treatment of cancer, curcumin a derivative of turmeric and the pigment that gives the curry spice turmeric its yellow-orange color may fit the bill. It's a natural compound that has been extensively researched, and has been found to have numerous health applications.
Like me, LaValley was trained in general medicine, but he's devoted a considerable amount of time to understanding the biochemical pathways that can support health nutritionally. In 1982, he participated in an exchange program to the People's Republic of China, where he got first-hand experience with the ancient practices of traditional Chinese medicine and acupuncture.
"One of the important messages that I learned there was that natural products, natural molecules, from plants and animals that are already available in nature, have been used by the Chinese for at least hundreds, probably thousands of years. That deeply changed my perspective in the world of medicine," he says.
"I came back to medical school, and thereafter, looked at how I could integrate the perspective of conventional pharmaceutical administration as well as natural extract, natural product administration."
Curcumin Has Potent Anticancer Activity
In 2005, he took a 75% sabbatical from clinical practice to immerse himself in the science of molecular biology, specifically the molecular biology of cancer. He also devoted approximately 9,000 to 9,500 hours building a relational database from the PubMed literature about the molecular biology of cancer.
One important lesson he learned through that venture is that the understanding of molecular biology can be applied across a range of diseases and symptoms described in the scientific literature. That knowledge can be applied by searching PubMed and other related databases, looking at the relevant molecular pathways involved.
..."In learning the molecular biology of cancer pathways, and in learning that what the evidence actually shows for the effect of natural product extracts on various relevant molecular targets in various cancers, we see that there's actually quite a large amount of evidence that supports using various molecules, natural products, and pharmaceuticals that are already approved and that have been around for a long time to affect anti-cancer activity along that pathway at that target.
Collagen and Gelatin Are Crucial for Optimal Health Articles
Collagen accounts for about 30% of the total protein in your body. One of its primary purposes is to provide structural support and strength to your tissues, such as skin, bones, tendons, ligaments and cartilage1,2,3 by allowing them to stretch while still maintaining tissue integrity.
As such, collagen is crucial for repairing soft tissue, muscle and connective tissue. Connective tissues include tendons, ligaments, cartilage and fascia, which tend to get weaker and less elastic with age. Connective tissue injuries are also problematic since theres very little blood supply in connective tissue, which slows down recovery.
Nearly one-third of the amino acids in collagen is glycine. It is also high in proline, hydroxyproline and alanine, which are the building blocks for the matrix of connective tissue. Your body uses the amino acids in collagen to rehab stressed areas and places in your body where its needed the most. Other lesser-known health benefits of collagen supplementation include:
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Deeper sleep due to its glycine content4 |
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Reduced joint pain and stiffness,5 including osteoarthritis pain6 |
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Improved gut health and digestion, thanks to the presence of glycine7 |
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Improved blood pressure and reduced cardiovascular damage8 |
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Improved glucose tolerance9 |
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Reduced inflammation and oxidative damage, as glycine inhibits the consumption of nicotinamide adenine dinucleotide phosphate (NADPH). NADPH is used as a reductive reservoir of electrons to recharge antioxidants once they become oxidized |
Important Differences Between Collagen and Red Meat
The chart below details the amino acid ratios of gelatin and collagen versus red meat (beef). As you can see, gelatin/collagen contain vastly more of the important amino acids to rebuild your connective tissue than beef. Since one-third of your bodys protein is collagen, it makes no sense to eat only muscle meat, as it will not provide enough amino acids to allow you to build strong connective t...
09:18
Discovery of potential phytochemicals from Carica papaya targeting BRCA-1 in breast cancer treatment. GreenMedInfo
PMID: Appl Biochem Biotechnol. 2023 Mar 29. Epub 2023 Mar 29. PMID: 36988843 Abstract Title: Discovery of Potential Phytochemicals from Carica papaya Targeting BRCA-1 in Breast Cancer Treatment. Abstract: The BRCA1 and BRCA2 are genes that encode a protein that ensures the integrity of DNA and prevents the unregulated cells from proliferating. Mutations in the sequence of these genes are associated with the birth of inherited breast cancers. The research for possible human breast cancer treatment remains a vital step in the drug development process. In this study, in silico investigations involving a computational method for the discovery of active phytochemicals from Carica papaya against the BRCA-1 gene were carried out. The in silico studies for these phytochemicals datasets as BRCA-1 breast cancer therapeutic agents showed promising results through pharmacokinetics and pharmacodynamics studies. The Carica papaya compounds were found to follow the rule of five and have good bioavailability. The ADMET and drug-likeness screening score of the identified ligands also recognized their potential as a promising drug candidate against BRCA-1 while the DFT also confirm better biological and chemical reactivity of Carica papaya compounds with excellent intra-molecular charge transfer between electron donor and electron acceptor site. The results of the molecular docking provided useful information on possible target-lead interactions, demonstrating that the newly developed leads showed a high affinity for BRCA-1 targets and might be investigated for further research.
09:09
Cytotoxic activity of the ethyl acetate extract of Iraqi Carica papaya leaves in breast and lung cancer cell lines. GreenMedInfo
PMID: Asian Pac J Cancer Prev. 2023 Feb 1 ;24(2):581-586. Epub 2023 Feb 1. PMID: 36853308 Abstract Title: Cytotoxic Activity of the Ethyl Acetate Extract of Iraqi Carica papaya Leaves in Breast and Lung Cancer Cell Lines. Abstract: UNLABELLED: The aim of this study was to evaluate the cytotoxic effect of the ethyl acetate fraction of Iraqi Carica papaya (C. papaya) in breast and lung cancer cell lines, MCF-7 and A549, respectively.METHODS: The ethyl acetate extract of Iraqi C. papaya leaves was prepared and tested for its phytochemical constitution. The 3-(4,5-dimethylthiazoline-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed in breast (MCF-7) and lung (A549) cells lines that were treated with different concentrations of ethyl acetate extract (3.125,6.25,12.5, 25, 50, and 100g/ml). After 72 hrs of treatment, cell viability was evaluated.RESULTS: The ethyl acetate extract of C. papaya showed considerable cytotoxic activity in the MCF-7 and A549 cell lines. The activity was dose-dependent; The half-maximum inhibitory concentration (IC50) values were 22.74g/ml and 8.674g/ml in the MCF-7 and A549 cell lines, respectively.CONCLUSION: The ethyl acetate fraction of Iraqi C. papaya leaves has potential anticancer activity in lung and breast cancer.
09:07
DHS Sought to Assign Social Credit Style "Risk Scores" to Social Media Users GreenMedInfo
Newly-obtained documents reveal.
09:04
DHS Sought to Assign Social Credit Style "Risk Scores" to Social Media Users GreenMedInfo
Originally published on www.reclaimthenet.org
by Cindy Harper
In a sharp spotlight on the interplay between national security and individual privacy, newly disclosed documents have unveiled that the Department of Homeland Security (DHS) entered into a contract with the University of Alabama at Birmingham (UAB) in 2018 to develop a project, dubbed "Night Fury," designed to analyze and assign "risk scores" to social media accounts.
08:59
Astragaloside IV alleviates 1-deoxysphinganine-induced mitochondrial dysfunction during the progression of chronic kidney disease. GreenMedInfo
PMID: Front Pharmacol. 2023 ;14:1092475. Epub 2023 Mar 24. PMID: 37033627 Abstract Title: Astragaloside IV alleviates 1-deoxysphinganine-induced mitochondrial dysfunction during the progression of chronic kidney disease through p62-Nrf2 antioxidant pathway. Abstract: Chronic kidney disease (CKD) can lead to significant elevation of 1-deoxysphingolipids (1-deoxySL). The increase of 1-deoxySL in turn can result in mitochondrial damage and oxidative stress, which can cause further progression of CKD.This study assessed the therapeutic effect of Astragaloside IV (AST) against 1-deoxySL-induced cytotoxicityand in rats with CKD. HK-2 cells were exposed to 1-deoxysphinganine (doxSA) or doxSA + AST. doxSA-induced mitochondrial dysfunction and oxidative stress were evaluated by immunostaining, real-time PCR, oxidative stress sensor, and transmission electron microscopy. The potential effects of AST on kidney damage were evaluated in a rat 5/6 nephrectomy (5/6 Nx) model of CKD.The findings ofexperiments showed that doxSA induced mitochondrial damage, oxidative stress, and apoptosis. AST markedly reduced the level of mitochondrial reactive oxygen species, lowered apoptosis, and improved mitochondrial function. In addition, exposure to AST significantly induced the phosphorylation of p62 and the nuclear translocation of Nrf2 as well as its downstream anti-oxidant genes. p62 knock-down fully abolished Nrf2 nuclear translocation in cells after AST treatment. However, p62 knock-down did not affect TBHQ-induced Nrf2 nuclear translocation, indicating that AST can ameliorate doxSA-induced oxidative stress through modulation of p62 phosphorylation and Nrf2 nuclear translocation.The findings indicate that AST can activate Nrf2 antioxidant pathway in a p62 dependent manner. The anti-oxidative stress effect and the further mitochondrial protective effect of AST represent a promising therapeutic strategy for the progression of CKD.
08:54
Astragaloside IV attenuates podocyte apoptosis through ameliorating mitochondrial dysfunction in diabetic kidney disease. GreenMedInfo
PMID: Free Radic Biol Med. 2023 Jul ;203:45-57. Epub 2023 Apr 6. PMID: 37030337 Abstract Title: Astragaloside IV attenuates podocyte apoptosis through ameliorating mitochondrial dysfunction by up-regulated Nrf2-ARE/TFAM signaling in diabetic kidney disease. Abstract: Defective antioxidant system as well as mitochondrial dysfunction contributes to the pathogenesis and progression of diabetic kidney disease (DKD). Nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated signaling is the central defensive mechanism against oxidative stress and therefore pharmacological activation of Nrf2 is a promising therapeutic strategy. In this study, using molecular docking we found that Astragaloside IV (AS-IV), an active ingredient from traditional formula of Huangqi decoction (HQD), exerted a higher potential to promote Nrf2 escape from Keap1-Nrf2 interaction via competitively bind to amino acid sites in Keap1. When podocyte exposed to high glucose (HG) stimulation, mitochondrial morphological alterations and podocyte apoptosis were presented and accompanied by Nrf2 and mitochondrial transcription factor A (TFAM) downregulation. Mechanistically, HG promoted a decrease in mitochondria-specific electron transport chain (ETC) complexes, ATP synthesis and mtDNA content as well as increased ROS production. Conversely, all these mitochondrial defects were dramatically alleviated by AS-IV, but suppression of Nrf2 with inhibitor or siRNA and TFAM siRNA simultaneously alleviated the AS-IV efficacy. Moreover, experimental diabetic mice exhibited significant renal injury as well as mitochondrial disorder, corresponding with the decreased expression of Nrf2 and TFAM. On the contrary, AS-IV reversed the abnormality and the Nrf2 and TFAM expression were also restored. Taken together, the present findings demonstrate the improvement of AS-IV on mitochondrial function, thereby resistance to oxidative stress-induced diabetic kidney injury and podocyte apoptosis, and the process is closely associated with activation of Nrf2-ARE/TFAM signaling.
08:46
Astragaloside IV targets PRDX6, inhibits the activation of RAC subunit in NADPH oxidase 2 for oxidative damage. GreenMedInfo
PMID: Phytomedicine. 2023 Jun ;114:154795. Epub 2023 Mar 29. PMID: 37030053 Abstract Title: Astragaloside IV targets PRDX6, inhibits the activation of RAC subunit in NADPH oxidase 2 for oxidative damage. Abstract: BACKGROUND: Radix Astragali Mongolici, as a traditional Chinese medicine, is widely used in the treatment of qi deficiency, viral or bacterial infection, inflammation and cancer. Astragaloside IV (AST), a key active compound in Radix Astragali Mongolici, has been shown to reduce disease progression by inhibiting oxidative stress and inflammation. However, the specific target and mechanism of action of AST in improving oxidative stress are still unclear.PURPOSE: This study aims to explore the target and mechanism of AST to improve oxidative stress, and to explain the biological process of oxidative stress.METHODS: AST functional probes were designed to capture target proteins and combined with protein spectrum to analyze target proteins. Small molecule and protein interaction technologies were used to verify the mode of action, while computer dynamics simulation technology was used to analyze the site of interaction with the target protein. The pharmacological activity of AST in improving oxidative stress was evaluated in a mouse model of acute lung injury induced by LPS. Additionally, pharmacological and serial molecular biological approaches were used to explore the underlying mechanism of action.RESULTS: AST inhibits PLA2 activity in PRDX6 by targeting the PLA2 catalytic triad pocket. This binding alters the conformation and structural stability of PRDX6 and interferes with the interaction between PRDX6 and RAC, hindering the activation of the RAC-GDI heterodimer. Inactivation of RAC prevents NOX2 maturation, attenuates superoxide anion production, and improves oxidative stress damage.CONCLUSION: The findings of this research indicate that AST impedes PLA2 activity by acting on the catalytic triad of PRDX6. This, in turn, disrupts the interaction between PRDX6 and RAC, thereby hindering the maturation of NOX2 and diminishing the oxidative stress damage.
08:33
Astragaloside IV alleviates stroke-triggered early brain injury. GreenMedInfo
PMID: Acta Cir Bras. 2023 ;38:e380723. Epub 2023 Mar 24. PMID: 36995819 Abstract Title: Astragaloside IV alleviates stroke-triggered early brain injury by modulating neuroinflammation and ferroptosis via the Nrf2/HO-1 signaling pathway. Abstract: PURPOSE: Stroke is an acute cerebrovascular disease. Astragaloside IV (AS-IV) is an active ingredient extracted from Astragalus membranaceus with an established therapeutic effect on central nervous system diseases. This study examined the neuroprotective properties and possible mechanisms of AS-IV in stroke-triggered early brain injury (EBI) in a rat transient middle cerebral artery occlusion (MCAO) model.METHODS: The neurological scores and brain water content were analyzed. 2,3,5-triphenyl tetrazolium chloride (TTC) staining was utilized to determine the infarct volume, neuroinflammatory cytokine levels, and ferroptosis-related genes and proteins, and neuronal damage and molecular mechanisms were evaluated by terminal deoxynucleotidyl transferase dutp nick-end labeling (TUNEL) staining, western blotting, and real-time polymerase chain reaction.RESULTS: AS-IV administration decreased the infarct volume, brain edema, neurological deficits, and inflammatory cytokines TNF-, interleukin-1(IL-1), IL-6, and NF-B, increased the levels of SLC7A11 and glutathione peroxidase 4 (GPX4), decreased lipid reactive oxygen species (ROS) levels, and prevented neuronal ferroptosis. Meanwhile, AS-IV triggered the Nrf2/HO-1 signaling pathway and alleviated ferroptosis due to the induction of stroke.CONCLUSIONS: Hence, the findings of this research illustrate that AS-IV administration can improve delayed ischemic neurological deficits and decrease neuronal death by modulating nuroinflammation and ferroptosis via the Nrf2/HO-1 signaling pathway.
08:25
Astragaloside IV induces the protective effect of bone marrow mesenchymal stem cells derived exosomes in acute myocardial infarction. GreenMedInfo
PMID: Biotechnol Genet Eng Rev. 2023 Mar 27:1-18. Epub 2023 Mar 27. PMID: 36971224 Abstract Title: Astragaloside IV induces the protective effect of bone marrow mesenchymal stem cells derived exosomes in acute myocardial infarction by inducing angiogenesis and inhibiting apoptosis. Abstract: Bone marrow mesenchymal stem cells (BMECs)-derived exosomes (MSC-Exo) can improve acute myocardial infarction (AMI). Astragaloside IV (AS-IV) has also been reported to have cardioprotective pharmacological effects. However, it is not entirely clear whether AS-IV can improve AMI by inducing MSC-Exo. BMSCs and MSC-Exo were isolated and identified, and we also established the AMI rat model and the OGD/R model with H9c2 cells. After MSC-Exo or AS-IV-mediated MSC-Exo treatment, cell angiogenesis, migration, and apoptosis were evaluated by tube formation, wound healing, and TUNEL staining. The cardiac function of the rats was measured by echocardiography. The pathological changes and collagen deposition in rats were also assessed with Masson and Sirius red staining. The levels of-SMA, CD31 and inflammatory factors were determined by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA)., AS-IV-mediated MSC-Exo can significantly enhance the angiogenesis and migration of H9c2 cells induced by OGD/R, and significantly reduce their apoptosis., AS-IV-mediated MSC-Exo can improve the cardiac function of rats, and attenuate pathological damage and collagen deposition in AMI model rats. In addition, AS-IV-mediated MSC-Exo can also promote angiogenesis and reduce inflammatory factors in rats with AMI. AS-IV-stimulated MSC-Exo can improve myocardial contractile function, myocardial fibrosis and angiogenesis, reduce inflammatory factors and induce apoptosis in rats after AMI.
08:11
Palmitoylethanolamide in the treatment of chronic pain. GreenMedInfo
PMID: Nutrients. 2023 Mar 10 ;15(6). Epub 2023 Mar 10. PMID: 36986081 Abstract Title: Palmitoylethanolamide in the Treatment of Chronic Pain: A Systematic Review and Meta-Analysis of Double-Blind Randomized Controlled Trials. Abstract: Chronic pain is a major source of morbidity for which there are limited effective treatments. Palmitoylethanolamide (PEA), a naturally occurring fatty acid amide, has demonstrated utility in the treatment of neuropathic and inflammatory pain. Emerging reports have supported a possible role for its use in the treatment of chronic pain, although this remains controversial. We undertook a systematic review and meta-analysis to examine the efficacy of PEA as an analgesic agent for chronic pain. A systematic literature search was performed, using the databases MEDLINE and Web of Science, to identify double-blind randomized controlled trials comparing PEA to placebo or active comparators in the treatment of chronic pain. All articles were independently screened by two reviewers. The primary outcome was pain intensity scores, for which a meta-analysis was undertaken using a random effects statistical model. Secondary outcomes including quality of life, functional status, and side effects are represented in a narrative synthesis. Our literature search identified 253 unique articles, of which 11 were ultimately included in the narrative synthesis and meta-analysis. Collectively, these articles described a combined sample size of 774 patients. PEA was found to reduce pain scores relative to comparators in a pooled estimate, with a standard mean difference of 1.68 (95% CI 1.05 to 2.31,= 0.00001). Several studies reported additional benefits of PEA for quality of life and functional status, and no major side effects were attributed to PEA in any study. The results of this systematic review and meta-analysis suggest that PEA is an effective and well-tolerated treatment for chronic pain. Further study is warranted to determine the optimal dosing and administration parameters of PEA for analgesic effects in the context of chronic pain.
08:00
Happy Hour Series with Lee Kessler: The White King Pt 4 Dr. Tenpenny
06-22-2023 Audio Version here: If you prefer to watch rather than listen, click on the video below: https://drtenpenny.b-cdn.net/2023/06-22-23-HHr-LeeKessler-Pt4.mp4 The White King book series are modern day allegories told through []
07:42
An Introduction to the Trieste Model of Mental Health Care, and Why It Matters Mad In America
From Vincenzo Passante/Psychiatry at the Margins: This approach is the basis (at least on paper) of the entire mental health system in Italy. So what is the Trieste model exactly?
. . . Anyone who is interested in the Trieste model should [get] to know its history, what the Basaglians always stood against in traditional psychiatry but also how they differed from other movements critical of psychiatry in the way they envisioned change.
While the Basaglian approach had significant influence outside Italy (for example in South America), it has been almost completely ignored in the US, and the UK has arguably been the most resistant country in the world to Basaglian practice since the 80s, with the beginning of a change of heart becoming evident only in very recent years. As a Basaglian who is originally from Trieste and now lives in the UK, I therefore face an up-hill task in influencing the English-speaking world on this subject.
. . . The system in Trieste is organized around a number of community mental health centers, the number of which varied across the years. Aside from a brief period for one of them currently, they are normally all open 24/7. These settings include beds for people who need them, but are also places where people can meet to pass some time with others, see a psychologist or another professional. They are therefore both crisis and not-crisis services, in line with the idea that freedom is therapeutic, which was one of the mottos of the revolution.
There is also a general hospital ward (the last time I visited, in April this year, it had 7 beds and 5 were occupied, but before the local government started cutting services, this place was often almost empty). There are no locked doors and no restraint is used across the whole mental health system. Conflict is resolved by means of negotiation and compromise. Help is not structured around treatment pathways based on a diagnosis (or alternative fixed conceptualizations), but on the persons whole life and needs across the board. This does not mean that disorders are not believed to exist, nor that technical interventions are not used, but that we put the illness in brackets and that we operate way beyond treatment. It means that intra-psychic problems exist within a whole life and societal context and that the context in itself can be part of both the problem and of the solution. The approach is to suspend judgement on the exact nature of a persons problem at the beginning of the relationship, and gradually help the person make sense of their life within a dialectical context.
These are the basic facts about the system and the vision of care that underpins it. The reaction from UK professionals to this picture is sometimes enthusiasm, sometimes curiosity and sometimes skepticism.
To address questions and concerns, I would like to briefly address a number of common objections I enc...
06:30
Empath Personalities Suffer From Overwhelming Emotional & Spiritual Exhaustion; Heres What It Looks Like And How To Shield Healthy Holistic Living
The term empathy finds its roots in two Greek words: em meaning in, and pathos meaning feeling. It denotes the capacity to understand and share the feelings of others. When it comes to individuals, empathic people demonstrate this emotional sensitivity at a higher level, often sensing the emotions and energies of those around them more intensely. Here are several traits that can help define an empathic person.
High Sensitivity
Empaths are typically more sensitive than the average person. They are deeply in tune with their own emotions and the emotions of others, often absorbing feelings from people around them. This heightened sensitivity can extend beyond emotions, including sensitivity to noise, smells, and large crowds.
Intuition
Empaths often exhibit strong intuitive abilities. They can sense the mood of a room when they walk in or intuit what someone is feeling without explicit communication. This allows them to understand others deeply, but it can also be draining if not managed effectively.
Emotional Absorption
Perhaps one of the most distinct characteristics of empaths is their tendency to absorb the emotions of others. They can often feel others pain, joy, and other emotions as if they were their own. This can make them excellent friends and partners as they genuinely understand and sympathize with the feelings of others.
Giving Nature
Empaths are naturally giving and nurturing. They tend to put others needs before their own and are driven by a desire to alleviate others suffering or enhance their happiness. This makes them great caregivers and healers, but it can also lead to feelings of exhaustion if they neglect their own...
06:26
Do physicians study vaccines? Absolutely, sorry anti-vaxxers Skeptical Raptor
The Skeptical Raptor, stalking pseudoscience in the internet jungle.
Anti-vaxxers love to state that physicians dont study vaccines. Of course, those same anti-vaxxers employ their Dunning-Kruger cognitive bias to claim that their 30 minutes of Google University research makes them the only experts in the world about vaccines. Using that logic, I could become an authorized Ferrari mechanic after watching a 30-minute YouTube video Read More Do physicians study vaccines? Absolutely, sorry anti-vaxxers
05:59
18 Anti-Parasitic Foods and Herbs To Add To Your Diet Healthy Holistic Living
Parasites are organisms that depend on a host for their survival. They attach themselves to or invade another living organism, feeding off their hosts nutrients and often causing health problems in the process. The host may experience a spectrum of health issues from mild gastrointestinal discomfort to debilitating diseases. These unwelcome guests can infiltrate your body in a variety of ways: consuming contaminated food or water, bites from insects, sexual contact, or even simply walking barefoot on contaminated soil.
However, the good news is that a careful choice of dietary intake can serve as a natural defense mechanism against such parasitic infections. This article aims to provide you with a detailed understanding of the best anti-parasitic foods, their health benefits, the scientific studies backing their use, and additional tips on how to incorporate them into your daily diet.
So how do you know if you have parasites? There are countless kinds of parasites that exist and many of them are so common, that you may even be unaware that some of the problems youre experiencing could be because of parasites.
Top 10 Signs You May Have A Parasite:
- Unexplained constipation, diarrhea, gas, or IBS symptoms
- Youve had travelers diarrhea while traveling internationally
- You have a history of food poisoning and are still dealing with digestive issues
- You have...
05:45
Basic vaccine chemistry for anti-vaxxers Skeptical Raptor
The Skeptical Raptor, stalking pseudoscience in the internet jungle.
One of the problems, among many, with anti-vaccine activists, is a complete misunderstanding of the basics of vaccine chemistry. People get scared of long chemical names, or chemicals themselves, and wonder if a vaccine might be dangerous. Setting aside the fact that vaccines undergo substantial and robust preclinical and clinical testing which has shown that Read More Basic vaccine chemistry for anti-vaxxers
04:27
Hone In On Healthy Blood Sugar Levels Dr. Tenpenny
One of the most frequent things we tell our patients at my clinics regards the importance of maintaining healthy blood sugar levels. Keeping blood sugar within the target range can []
03:00
Psychiatric and Behavioral Genetics at the Crossroads: Mary Boyle Interviews Jay Joseph Mad In America
In 2004 I was interviewed by Craig Newnes for The Psychologist, a journal published by the British Psychological Society. The occasion was the publication of my first book, The Gene Illusion: Genetic Research in Psychiatry and Psychology Under the Microscope. Upon publishing my fourth book, Schizophrenia and Genetics: The End of An Illusion (2023), psychologist/author Mary Boyle invited me for a new interview to discuss the book and the changes that have occurred in behavioral science genetic research since 2004. Marys questions, and my answers, are found below. Jay Joseph
Mary Boyle: Jay, you were interviewed in 2004 about your book The Gene Illusion. It was a detailed critique of genetic research on schizophrenia, IQ and criminality and you concluded that there was little if any scientifically valid evidence in support of genetic influences on human behavioral differences. Your latest book, Schizophrenia and Genetics: The End of An Illusion has just been published. It, and your many other publications, show that a lot has happened in psychiatric and behavioral genetics research over the last 20 years. Well be talking about what has and hasnt changed and whether your earlier conclusion still stands. But first, tell us how you became interested in this area and what keeps you engaged with it.
Jay Joseph: I became interested in the genetics of schizophrenia topic as a U.S. clinical psychology graduate student in the mid-1990s. The arguments fascinated me, and because I saw the genetic argument as weak, it was stunning to hear that the debate had been largely closed in favor of genetics by the 1980s. My desire to learn more about genetic research led me...
02:00
EPA Breaking the Law to Help Pesticide Companies Alliance for Natural Health USA Protecting Natural Health
The agency is giving pesticide-coated seeds a pass as they poison local communities and destroy ecosystems. Action Alert!
Over the last few months, weve been reporting on the health and environmental catastrophe linked to the use of pesticide-coated seeds, which were utilized to produce ethanol at a plant in Mead, Nebraska.
Incredibly, the use of pesticide-coated seeds, both in ethanol production and to grow food, is completely legal, and the EPA has refused to regulate these toxic seeds, probably because it does not want to step on the toes of big agribusinesses. The agency is now being sued by advocacy groups for violating its own pesticide laws. Think about this: the agency charged with protecting the environment and human health needs to be challenged in court to protect the environment and human health over industry profits. It is a sad commentary on state of affairs in Washington, but we must help hold the agencys feet to the fire.
The lawsuit, brought forth by the Center for Food Safety and the Pesticide Action Network, seeks to close a loophole at the EPA that allows seeds coated with neonicotinoid (neonic for short) pesticides to evade regulation under federal pesticide laws. The groups argue that pesticide-coated seeds should be regulated like other pesticides under the Federal Insecticide, Fungicide and Rodenticide Act (FIFRA), which would trigger closer regulatory scrutiny, in particular an analysis of the seeds benefits versus their costs to the environment and human health. Notably, if neonic-coated seeds were regulated under FIFRA, the EPA would have to show that the seeds do not cause unreasonable or adverse effects to the environment. As we will discuss below, this would be a heavy lift for the EPA, which is likely the reason the agency is fighting to keep the loophole open.
The EPA claims that the seeds fall under an exemption to FIFRA called the Treated Article Exception. These are items treated with pesticides to protect the item itself. The...
01:53
ANHs Free Speech Fight Gains Momentum Alliance for Natural Health USA Protecting Natural Health
ANH-USAs efforts to get to the bottom of the Federal Trade Commissions (FTC) latest free speech attack is picking up steam and getting important coverage in the media. Action Alert!
Last week, we reported that we submitted a Freedom of Information Act (FOIA) request to learn the real motivations for the FTCs attack on free speech in the natural products sector. Our FOIA request stems from concerns that the FTCs recent guidance will have the effect of limiting consumer access to vital scientific information about the benefits of dietary supplements and other natural products. We believe the agency is poised to start cracking down on health claims related to nutrients, and we want to learn how the new FTC policy was generated, which could potentially serve as the basis for a legal challenge to the agencys overreach.
Independent news outlets have taken notice of our efforts. You can see coverage of our important work in this area here, here, and here.
Were facing a government-led campaign to conceal the remarkable truth about the healing and disease-preventing powers of foods and nutrients, ultimately misleading the public so that they spend their money on drugs rather than natural supplements.
Lets keep up the momentum. Help us push back against the governments censorship campaign and support free speech!
Action Alert! Write to Congress and the FTC, telling them to complete ANHs FOIA request. Please send your message immediately.
The post ANHs Free Speech Fight Gains Momentum first appeared on Alliance for Natural Health USA - Protecting Natural Health.
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Thursday, 22 June
12:39
Algonac City Council approves planning commission reappointments. Water plant items also discussed Fluoride Action Network
Algonac City Council members approved reappointments to the planning commission and discussed several water plant items at a June 20 meeting.
Mayor Rocky Gillis said four planning commission board members had terms expiring July 17 and would like to be considered for reappointment: Rich Arpan, Amanda Hass, Brian Tideswell and Mark Thompson.
The boards and commissions appointment policy approved by city council in December 2015 states that no new applicants would be sought out in the case of a requested reappointment, unless by majority of the council, Gillis said.
Council member Corey Blair made a motion to approve the reappointments of Arpan, Hass, Tideswell and Thompson to the planning commission for three-year terms ending July 1, 2026.
He thanked each of them.
Their reappointment makes everything a lot smoother, especially with everybody still within the group and been on the group for a while, so I commend them and say thank you, he said.
Theyre all good people, council member Michael Bembas said.
Gillis agreed, and the motion was unanimously approved.
Council members also thanked and congratulated those reappointed to the planning commission during the council comment portion of the meeting.
It takes a lot to volunteer for commissions, especially one as important as the planning commission, council member Dawn Davey said.
Mayor Pro Tem Raymond Martin commented that the four are volunteers and give up their time to serve on the commission.
I would also to thank our reappointments, Gillis said. It makes our job much easier and we dont have to go out and search for candidates, so we appreciate their service.
Water Plant Items
The council also discussed several water plant items.
Were being asked to approve water plant fluoride probe replacement, Gillis said. The water plant is required to test the amount of fluoride in both raw water and drinking water. The current probe is no longer working.
He said the city received a quote from USA Blue Book for a new fluoride probe and associated solutions.
Davey made a motion to approve water plant fluoride probe replacement from USA Blue Book in the amount of $1,118.14.
City Manager Denice Gerstenberg said a new fluoride probe was ordered as soon as it was discovered that the previous one was not working anymore.
Bembas suggested ordering a second probe so that if one stops working, there is a backup.
I would like to ask the public services superintendent if that would be beneficial for him and then get back with you, Gerstenberg said.
The motion was unanimously approved.
The council also unanimously approved a motion to approve a one-year maintenance contract for 24-hour monitoring of chlorine levels to Hach in the amount of $4,323.
The water plant has three CL-17 online chlorine analyzers and one bencht...
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Wednesday, 21 June
12:36
Andrew John Rugg-Gunn named 2023 recipient of IADR Gold Medal Fluoride Action Network
Alexandria, VA, USA The International Association for Dental Research (IADR) has announced Andrew John Rugg-Gunn as the 2023 recipient of the IADR Gold Medal. Rugg-Gunn, from the Borrow Foundation, Hampshire, England, was recognized during the Opening Ceremonies of the 101st General Session of the IADR, which was held in conjunction with the 9th Meeting of the Latin American Region and the 12th World Congress on Preventive Dentistry, that took place on June 21, 2023, in Bogot, Colombia.
Rugg-Gunn has had a major influence on the recognition of the impact of diet and oral health with his service on the Scientific Advisory Committee on Nutrition (SACN), his advice and influence on WHO policy, and his influential role in pediatric dentistry in the promotion of fluorides.
As head of the department of Child Dental Health, within Newcastle University during the late 1990s, Rugg-Gunn influenced the careers of scientists within the department and dental school. Two of these went on to become IADR Presidents Paula Moynihan and Angus Walls.
For the past 30+ years, Rugg-Gunn has had an advisory role with the World Health Organization (WHO). He served as an advisor regarding revisions of Oral Health Surveys, Basic Methods (now in its 5th edition), and the revision of Fluorides and Oral health (Technical Report, 1994), published in 2016. He also co-authored the WHO publication Milk Fluoridation for the Prevention of Dental Caries, (2009), and two WHO documents on early childhood caries. Additionally, he was part of a WHO team invited to Beijing in 2000 by the Chinese Ministry of Health to advise on national fluoride policy.
The IADR Gold Medal is the highest recognition bestowed by the IADR. It is presented to a previous recipient of an IADR Distinguished Scientist Award who later builds on their original scientific achievements to more broadly impact science, health research, or population health through the expansion of their field into other disciplines, or through higher administrative positions in academia, government, non-profit, or private industry.
About IADR
The International Association for Dental Research (IADR) is a nonprofit organization with a mission to drive dental, oral, and craniofacial research for health and well-being worldwide. IADR represents the individual scientists, clinician-scientists, dental professionals, and students based in academic, government, non-profit and private-sector institutions who share our mission. Learn more at www.iadr.org.
*Original full-text article online at: https://www.eurekalert.org/news-releases/992454
Tuesday, 20 June
12:46
Fluoridation could distract from other oral health issues Fluoride Action Network
MHK fears adding substance to drinking water would mean less emphasis on other dental factors
Adding fluoride to the Islands drinking water could distract from other aspects of oral health.
Thats the fear of one of the members of a Tynwald committee thats investigated dental issues among Manx children.
The committee initially suggested a twice-yearly fluoride varnish for children, but the Council of Ministers put forward an amendment seeking a study into fluoridation instead.
With the Manx parliament set to vote on the issue at this months sitting, Garff MHK Daphne Caine says its not the solution to all dental problems:
*Original full-text article online at: https://www.manxradio.com/news/isle-of-man-news/fluoridation-could-distract-from-other-oral-health-issues/